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Table 3 List of genes spotted twice on the microarray filter which evinced a significant change in their mRNA expression in TGFβ-treated T84 cells compared to T84 cells grown solely in collagen I gel (= TGFβ-treated vs. control).

From: Gene expression in TGFbeta-induced epithelial cell differentiation in a three-dimensional intestinal epithelial cell differentiation model

Gene ontology class

Gene name

GenBank no

chromosomal location

TGFβ-treated vs. control

    

ratio

SD

ratio

SD

A

FDPS: farnesyl diphosphate synthase (farnesyl pyrophosphate synthetase, dimethylallyltranstransferase, geranyltranstransferase)

T66907

T65907

1q22

0.29↓

(±0.24)

0.57↓

(±0.45)

A

GRP58: glucose regulated protein, 58 kD,

R33030

15q15

0.56↓

(±0.12)

0.60↓

(±0.16)

C

BAP1: BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase)

H09065

3p21.31-p21.2

0.72↓

(±0.19)

0.68↓

(±0.17)

D

DRAP1: DR1-associated protein 1

AA421977

AA406285

11q13.3

0.53↓

(±0.22)

0.60↓

(±0.19)

D

AEBP1: AE binding protein 1

AA490462

AA490684

7p13

0.48↓

(±0.34)

0.60↓

(±0.32)

D

ZNF161: zinc finger protein 161

AA232647

17q23.3

0.71↓

(±0.13)

0.75↓

(±0.11)

E

IGF2: insulin-like growth factor 2 (somatomedin A)

N54596

11p15.5

0.57↓

(±0.16)

0.63↓

(±0.17)

E

MAST1: microtubule associated serine/threonine kinase 1

AA479623

19p13.2

0.51↓

(±0.18)

0.59↓

(±0.48)

E

EGFR: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)

R35665

W48713

7p12

0.47↓

(±0.04)

0.75↓

(±0.13)

E

EPHA1: ephrin receptor EphA1

N90246

7q34

0.66↓

(±0.10)

0.63↓

(±0.07)

E

RNTRE: related to the N terminus of tre

AA281057

AA281137

10p13

0.78↓

(±0.25)

0.71↓

(±0.21)

E

EFNB2: ephrin-B2

AA461424

AA461108

13q33

0.77↓

(±0.20)

0.79↓

(±0.06)

F

UBE2N: ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)

AA490124

12q21.33

0.76↓

(±0.16)

0.79↓

(±0.17)

I

ATP6V0A1: ATPase, H+ transporting, lysosomal V0 subunit a isoform 1

AA427472

17q21

0.65↓

(±0.02)

0.62↓

(±0.08)

  1. Genes are sorted by functional classification. A: Cellular metabolism (GO:0044237), Metabolism (other than energy metabolism), C: Nucleic acid binding (GO:0003676), D: Transcription regulator activity (GO:0030528), E: Signal transducer activity (GO:0004871), Signal transduction (GO:0007165) and Cell communication (GO:0007154), F: Cellular macromolecule metabolism (GO:0044260), I: Transport (GO:0006810), Downward arrow indicates down-regulated mRNA expression, bold indicates genes that have significant alteration in gene expression. Ratio is mean value calculated from three separate microarray experiments; SD is the standard deviation between separate experiments.
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BMC Genomics

ISSN: 1471-2164

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