|
Gene ontology class
|
name
|
GenBank no
|
microarray TGFβ-treated vs. control
|
RT-PCR TGFβ-treated vs. control
|
microarray IMR-treated vs. control
|
RT-PCR IMR-treated vs. control
|
|---|
| | | |
ratio
|
SD
|
ratio
|
SD
|
ratio
|
SD
|
ratio
|
SD
|
|---|
|
A
|
GM2A
|
AA453978
|
0.43↓
|
(±0.26)
|
0.39↓
|
(±0.38)
|
0.75↓
|
(±0.47)
|
0.29↓
|
(±0.19)
|
|
A
|
DIO2
|
R62242
|
0.49↓
|
(±0.38)
|
0.01↓
|
(±0.02)
|
0.99
|
(±0.76)
|
1.12
|
(±1.6)
|
|
D
|
ROK1
|
W73792
|
0.47↓
|
(±0.25)
|
0.32↓
|
(±0.13)
|
0.80
|
(±0.47)
|
1.34↑
|
(±0.23)
|
|
E
|
DRPLA
|
H08642
|
0.65↓
|
(±0.42)
|
0.71↓
|
(±0.12)
|
0.80↓
|
(±0.53)
|
0.66↓
|
(±0.24)
|
|
E
|
PLCG1
|
R76365
|
0.48↓
|
(±0.23)
|
0.33↓
|
(±0.26)
|
0.81↓
|
(±0.48)
|
0.47↓
|
(±0.28)
|
|
F
|
VRK2
|
AA490617
|
0.89↓
|
(±0.16)
|
0.04↓
|
(±0.03)
|
0.78↓
|
(±0.22)
|
0.24↓
|
(±0.34)
|
|
F
|
CRYAB
|
AA504943
|
0.40↓
|
(±0.08)
|
0.71↓
|
(±0.30)
|
0.72↓
|
(±0.30)
|
0.62↓
|
(±0.13)
|
|
F
|
PSMB
|
AA070997
|
0.76↓
|
(±0.16)
|
0.30↓
|
(±0.15)
|
0.99
|
(±0.12)
|
0.51↓
|
(±0.24)
|
|
H
|
KRT19
|
AA464250
|
13.90
|
(±16.73)
|
2.43↑
|
(±0.83)
|
19.95
|
(±12.99)
|
1.14
|
(±0.32)
|
- TGFβ-differentiated T84 cells compared to T84 cells grown solely in collagen I gel (= TGFβ-treated vs. control) and T84 cells differentiated by soluble factors secreted by mesenchymal cells compared to T84 cells grown solely in collagen I gel (= IMR-treated vs. control). Genes are sorted by functional classification. A: Cellular metabolism (GO:0044237), Metabolism (other than energy metabolism), D: Transcription regulator activity (GO:0030528), E: Signal transducer activity (GO:0004871), Signal transduction (GO:0007165) and Cell communication (GO:0007154), F: Cellular macromolecule metabolism (GO:0044260), H: Organelle organization and biogenesis (GO:0006996I). Upward arrow denotes up-regulated mRNA expression, downward arrow down-regulated mRNA expression, bold indicates genes that have significant alteration in gene expression. Ratios are the mean values calculated from three separate microarray experiments and five separate RT-PCR experiments, the calculated standard deviations (SD) between separate experiments is in the brackets.
