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Table 4 Microarray data and LightCycler RT-PCR mRNA levels as ratios of VRK2, GM2A, CRYAB, TAGLN, DRPLA, DIO2, PSMB, ROK1, PLCG and KRT19.

From: Gene expression in TGFbeta-induced epithelial cell differentiation in a three-dimensional intestinal epithelial cell differentiation model

Gene ontology class name GenBank no microarray TGFβ-treated vs. control RT-PCR TGFβ-treated vs. control microarray IMR-treated vs. control RT-PCR IMR-treated vs. control
    ratio SD ratio SD ratio SD ratio SD
A GM2A AA453978 0.43↓ (±0.26) 0.39↓ (±0.38) 0.75↓ (±0.47) 0.29↓ (±0.19)
A DIO2 R62242 0.49↓ (±0.38) 0.01↓ (±0.02) 0.99 (±0.76) 1.12 (±1.6)
D ROK1 W73792 0.47↓ (±0.25) 0.32↓ (±0.13) 0.80 (±0.47) 1.34↑ (±0.23)
E DRPLA H08642 0.65↓ (±0.42) 0.71↓ (±0.12) 0.80↓ (±0.53) 0.66↓ (±0.24)
E PLCG1 R76365 0.48↓ (±0.23) 0.33↓ (±0.26) 0.81↓ (±0.48) 0.47↓ (±0.28)
F VRK2 AA490617 0.89↓ (±0.16) 0.04↓ (±0.03) 0.78↓ (±0.22) 0.24↓ (±0.34)
F CRYAB AA504943 0.40↓ (±0.08) 0.71↓ (±0.30) 0.72↓ (±0.30) 0.62↓ (±0.13)
F PSMB AA070997 0.76↓ (±0.16) 0.30↓ (±0.15) 0.99 (±0.12) 0.51↓ (±0.24)
H KRT19 AA464250 13.90 (±16.73) 2.43↑ (±0.83) 19.95 (±12.99) 1.14 (±0.32)
  1. TGFβ-differentiated T84 cells compared to T84 cells grown solely in collagen I gel (= TGFβ-treated vs. control) and T84 cells differentiated by soluble factors secreted by mesenchymal cells compared to T84 cells grown solely in collagen I gel (= IMR-treated vs. control). Genes are sorted by functional classification. A: Cellular metabolism (GO:0044237), Metabolism (other than energy metabolism), D: Transcription regulator activity (GO:0030528), E: Signal transducer activity (GO:0004871), Signal transduction (GO:0007165) and Cell communication (GO:0007154), F: Cellular macromolecule metabolism (GO:0044260), H: Organelle organization and biogenesis (GO:0006996I). Upward arrow denotes up-regulated mRNA expression, downward arrow down-regulated mRNA expression, bold indicates genes that have significant alteration in gene expression. Ratios are the mean values calculated from three separate microarray experiments and five separate RT-PCR experiments, the calculated standard deviations (SD) between separate experiments is in the brackets.