Figure 4

Analysis of the pheromone-induced gene SPBC4.01. A. Expression of SPBC4.01 in P cells (left half, red bars) and M cells (right half, blue bars). Cells were either growing in medium with nitrogen (+), without nitrogen (-), or without nitrogen in the presence of M factor (-/M) or P-factor (-/P). At the different growth conditions, the level of SPBC4.01 RNA was quantitated relative to act1 RNA by real time PCR. B. Sporulation defect of ΔSPBC4.01 cells. The picture shows iodine staining of h⁹⁰ wild type and h⁹⁰ ΔSPBC4.01 colonies grown on the same MSA plate. C. Micrographs of h⁹⁰ wild type and h⁹⁰ ΔSPBC4.01 cells showing examples of abnormal mating and sporulation in the latter.