Analysis of SPAC3F10.09 expression. A. Expression profile of SPAC3F10.09 relative to act1 (see Figure 4 for experimental details). B. Schematic representation of the chromosomal region encompassing SPAC3F10.09 and map3. The open reading frames of the two genes are shown as boxes, with arrows after the gene names indicating the direction of transcription. The three introns in SPAC3F10.09 are shown in grey. The position of the two SPAC3F10.09 specific primers are indicated with half-arrows. The position of the mapped 3'polyA tail of the map3 transcript in the second intron of SPAC3F10.09 is at the bend of the dotted line. C. Strand-specific RT PCR analysis of SPAC3F10.09 showing expression from, respectively, the SPAC3F10.09 strand (left half) or the map3 strand (right half) on RNA from h+ cells under the growth conditions indicated below.