Deep and comparative analysis of the mycelium and appressorium transcriptomes of Magnaporthe grisea using MPSS, RL-SAGE, and oligoarray methods

Table 1 MPSS and RL-SAGE libraries from mycelial and appressorial stages of 70-15 strain of M. grisea

Library	MPSS		RL-SAGE
Tissue	Appressoria	Mycelia	Mycelia
Growth stage	24-hr after germination	96-hr liquid culture	96-hr liquid culture
Anchoring enzyme and tag size	GATC (Dpn II) + 13 bases	GATC (Dpn II) + 13 bases	CATG (Nla III) + 17 bases
No. of genomic virtual tags ¹	286,874	286,874	355,837
No. of total tags per library	1,459,146	1,326,720	245,873
No. of unique tags per library	16,427	20,299	51,925
No. of copies per tag	2 to 28,845	1 to 30,910	1 to 7,383
No. of significant tags ²	12,927	12,531	16,580
No. of non-significant tags ³	3,500	7,768	35,345
No. of significant antisense tags ⁴	653 (5.1%)	1,825 (14.6%)	605 (3.6%)

¹Genomic tags were extracted from the M. grisea draft genomic sequence as described in the text.
²Significant tags are those with ≥ 2 copies per tag in RL-SAGE and ≥ 4 copies per tag in MPSS.
³Non-significant tags are those with one copy per tag in RL-SAGE and ≤ 3 copies per tag in MPSS.
⁴Significant antisense tags are those with ≥ 2 copies per tag in RL-SAGE and ≥ 4 copies per tag in MPSS matched to the annotated genes

ISSN: 1471-2164