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Table 1 MPSS and RL-SAGE libraries from mycelial and appressorial stages of 70-15 strain of M. grisea

From: Deep and comparative analysis of the mycelium and appressorium transcriptomes of Magnaporthe grisea using MPSS, RL-SAGE, and oligoarray methods

Library MPSS   RL-SAGE
Tissue Appressoria Mycelia Mycelia
Growth stage 24-hr after germination 96-hr liquid culture 96-hr liquid culture
Anchoring enzyme and tag size GATC (Dpn II) + 13 bases GATC (Dpn II) + 13 bases CATG (Nla III) + 17 bases
No. of genomic virtual tags 1 286,874 286,874 355,837
No. of total tags per library 1,459,146 1,326,720 245,873
No. of unique tags per library 16,427 20,299 51,925
No. of copies per tag 2 to 28,845 1 to 30,910 1 to 7,383
No. of significant tags 2 12,927 12,531 16,580
No. of non-significant tags 3 3,500 7,768 35,345
No. of significant antisense tags 4 653 (5.1%) 1,825 (14.6%) 605 (3.6%)
  1. 1Genomic tags were extracted from the M. grisea draft genomic sequence as described in the text.
  2. 2Significant tags are those with ≥ 2 copies per tag in RL-SAGE and ≥ 4 copies per tag in MPSS.
  3. 3Non-significant tags are those with one copy per tag in RL-SAGE and ≤ 3 copies per tag in MPSS.
  4. 4Significant antisense tags are those with ≥ 2 copies per tag in RL-SAGE and ≥ 4 copies per tag in MPSS matched to the annotated genes