Figure 3
From: Quantitative analysis of cell-type specific gene expression in the green alga Volvox carteri
Comparison of gene expression of six target genes in gonidia versus somatic cells by quantitative real-time RT-PCR. Amplification curves for A) actA (internal control for the 2-ΔΔCt method), B) gon167, C) ssgA, D) rpl37, E) fer1, and F) regA. The target-specific fluorescence signal of SYBR Green fluorescence emission (detection range 515–545 nm) is plotted against the number of PCR cycles. Curves of gonidial RT-PCRs are given in red, somatic RT-PCRs in blue. All real-time RT-PCR experiments were carried out in triplicate, and a mean amplification curve was generated for each cell-type. The threshold level is given by a broken, horizontal line. The cycle at which the mean amplification curve of gonidial or somatic real-time RT-PCRs crosses the threshold (Ct value) is indicated by a broken, vertical line.