Figure 3

Mismapped clones. A. Array-CGH profile of UOK115 cell line. BAC/PACs covering chr3 are displayed in order on the X-axis from telomere of short arm (left), to telomere of long arm (right). Controls derived from chrX are plotted between the vertical lines right to chr3. Controls from autosomes are plotted to the right of the chrX controls. The Y-axis denotes the value of normalized fluorescence ratio (NFR) for each BAC/PAC clone (see Methods). The profile identifies 3p loss with ANILFR ± 1 sd; 0.7 ± 0.07. Two clones highlighted by circles, display outstanding fluorescence ratio than the neighbouring clones. On the plot arrows indicate real localizations revealed by FISH (RP11-129G16 locates at 3q12, clone RP11-732M7 at 3p24-25). Triangles indicate clones, which are part of segmental duplications identified by BLASTN. B. FISH image of normal metaphase with clones: RP11-994B16 (3q21.3), red, and the real localization of RP11-732M7, green, at 3q24-25.