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Table 1 Summary of the selected SNPs and the respective method of genotyping. Gene/SNP, rs number given when available, aminoacid change or UTR. Primer set: for the assyas developed for this study or otherwise as referred to original publication. Method of analysis: platform and assay selection.

From: Multilocus analysis of SNP and metabolic data within a given pathway

Gene/SNP Primer set Method of analysis
CYP11A1, repeat f(5'-6-FAM-GTC-AGC-TGT-ACT-GAA-TTA-CAG-CC-3') r(5'-TCT-TGC-CAG-CTT-GGG-CAA-CAT-ACG-3' Fragment analysis ABI310, (Applied Biosystems)
CYP17, rs 743572 f 5'-CAT-TCG-CAC-CTC-TGG-AGT-3' r 5'-GGC-TCT-TGG-GGT-ACT-TG-3' RLFP Msp A1 (Promega),
CYP19 3'UTRSNP1, rs10046 5'-ATA TTC TGG CAA CTG TCT-3' 5'-GAG GAT GAC ACT ATT GGC-3' BDT (Big Dye Terminator) sequencing ABI310 (Applied Biosystems)
CYP19 3'UTRSNP2, rs4646 5'-GAC AGT GTG TTG AGA GCA TAC AGA-3' 5'-TTC TTC ACC GAC TAT TTC TC-3' BDT (Big Dye Terminator) sequencing ABI310 (Applied Biosystems)
CYP19 3'UTRSNP3, rs2255192 5'-CAT TGA ATC ATT GTA TGT GGT CAT-3' 5'-GGA AAT GGG ATG GAA ATA GAT TAC A-3' BDT (Big Dye Terminator) sequencing ABI310 (Applied Biosystems)
HSD-A3T, rs3138620 5'-6-FAM CAG TAC TAA AGG CCC TAT TAT CAA A-3' 5'-AGG CTG CAG TGA GTC CAG AT-3' Fragment analysis ABI310, (Applied Biosystems)
HSD-DEL, rs8191194 5'-6-FAM GTG ACC CAC GAA ACA CAG G-3' 5'-CAG AAG GTG AAG AAC TCA TCC A-3' Fragment analysis on a ABI310, (Applied Biosystems)
CYP1B1 R48G 5'-GCACCCCTGAGTGTCACGCC-3', 5'-TTCCAGTGCTCCGAGTAGTG-3' RFLP, Rsr II. (Fermentas)
CYP1B1 A119S 5'-GCACCCCTGAGTGTCACGCC-3', 5'-TTCCAGTGCTCCGAGTAGTG-3' RLFP, NgoM IV (New England BioLabs)
CYP1B1 V432L, rs1056836 5'-CCAGCCCAACCTGCCCTATG-3' 5'-CCAGGATGGAGATGAAGA-3' RLFP, Eco577 (Fermentas)
CYP1A1m1 T3801C Cascorbi et [19]. See reference
CYP1A1m2 I462V, rs1048943 Cascorbi et [19]. See reference
CYP1A1mS T3205C Cascorbi et [19]. See reference
CYP1A1m4 T461N, rs1799814 Cascorbi et [19]. See reference
COMT, rs4680 Matsui et al. 2000, [20]. See reference
GSTM1 Kristensen et al 1998 [31]. See reference
GSTT1 Kristensen et al 1998[31]. See reference
GSTP1 Kristensen et al 1998[31]. See reference
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