Analysis of PpSP15 family of sandfly salivary proteins. (A) Multiple sequence alignment of PpSP15 salivary proteins from Phlebotomus argentipes (Pag), Phlebotomus ariasi (Par), Phlebotomus perniciosus (Ppe) and Lutzomyia longipalpis (LJ). Sequences were aligned using ClustalX and manually refined using BioEdit sequence-editing software. (B) Phylogenetic tree analysis of PpSP15 salivary proteins from these four sandflies. Phylogenetic analysis was conducted on protein alignments using Tree Puzzle version 5.2 by maximum likelihood using quartet puzzling, automatically estimating internal branch node support (1000 replications).