Analysis of D7-related proteins. A) Multiple sequence analysis of D7 proteins from the salivary glands of Phlebotomus argentipes (Pag), Phlebotomus ariasi (Par), Phlebotomus perniciosus (Ppe) and Lutzomyia longipalpis (LJ). Sequences were aligned using ClustalX and manually refined using BioEdit sequence-editing software. (B) Phylogenetic tree analysis of D7 salivary proteins from P. argentipes (Pag), P. ariasi (Par), P. perniciosus (Ppe), L. longipalpis (LJ), P. papatasi, Aedes aegypti, Ae. albopictus, Anopheles gambiae, An. Arabiensis, An. Darlingi, An. Stephensi and Culex quinquefasciatus. Phylogenetic analysis was conducted on protein alignments using Tree Puzzle version 5.2 by maximum likelihood using quartet puzzling, automatically estimating internal branch node support (1000 replications).