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Table 1 Summary of pyrosequence data from the Soudan Mine

From: Using pyrosequencing to shed light on deep mine microbial ecology

 

Red Sample

Black Sample

Number of Sequences

334,386

388,627

Total Length of Sequences

35,439,683 bp

38,502,057 bp

Average Length of Sequences

106.0 bp

99.1 bp

Average Quality Score1

26.2

25.8

Skew2

2.53

2.44

  1. 1 The quality score of each base was provided by 454 Life Sciences, and is analogous to the Phred score of Sanger Sequencing methods [26]. The value cited here is the average of the mean quality score per sequence.
  2. 2The skew was calculated by comparing the dinucleotide frequencies within each library. A similar analysis performed on Bacterial and Eukaryotic genomes sampled at random yielded entropies of 2.63 and 2.66 respectively.
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BMC Genomics

ISSN: 1471-2164

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