Table 1 Overview of the two microarray platforms and TaqMan® GeneExpression Assay based real-time PCR
| Â | Applied Biosystems | Agilent | TaqMan Gene Expression Assays |
|---|---|---|---|
Platforms | Human Genome Survey Microarray | Human Whole Genome Arrays | TaqMan Expression Assays |
Technology | Hybridization | Comparative Hybridization | 5' Nuclease Chemistry & Real Time PCR |
Probe (bases) | 60mer | 60mer | TaqMan Primer & Probes |
Substrate | Nylon | Glass slide | - |
Deposition | Contact Spotting | In-situ Ink Jet Printing | - |
Detection | One-color Chemiluminescence | Two-color Cy3/Cy5 Fluorescence | One-color FAM Fluorescence |
Software | 1700 Chemiluminescence Microaray Analyzer Software v 1.1 | Feature Extraction A 7.5.1 | SDS 2.1 |
Total Probes | 33,096 probes | 44,000 probes | 1375 Selected Targets |