Table 1 Relation between RSCU' and measures of expression for all codons of amino acids of degeneracy greater than one. For each codon, only the genes in which the corresponding amino acid has a frequency of at least 10 were considered. Major codons are underlined. ^w indicates G/U wobble (by Lander et al. 2001). * indicates translationally weak codons. The (+) sign indicates that the average RSCU' in class of 25% lowly expressed genes is significantly lower than the average RSCU' in class of 25% highly expressed genes, while the (-) sign represents the opposite. N.S. indicates that the RSCU' is not significantly different

Ala

GCA

9

N.S.

+

GCC^W

0

N.S.

-

GCG*

5

-

-

GCT

29

+

+

Arg2

AGA

6

N.S.

+

AGG

5

-

-

Arg4

CGA

6

N.S.

+

CGC^W

0

N.S.

-

CGG

5

N.S.

N.S.

CGT

7

N.S.

N.S.

Asn

AAC

28

N.S.

-

AAT^W

1

N.S.

N.S.

Asp

GAC

18

-

-

GAT^W

0

+

+

Cys

TGC

30

N.S.

N.S.

TGT^W

0

N.S.

N.S.

Gln

CAA*

11

-

-

CAG

21

+

+

Glu

GAA

12

N.S.

+

GAG

13

N.S.

-

Gly

GGA

9

N.S.

+

GGC

15

+

N.S.

GGG*

7

-

-

GGT^W

0

+

+

His

CAC

11

N.S.

N.S.

CAT^W

0

N.S.

N.S.

Ile

ATA*

5

-

-

ATC^W

5

+

N.S.

ATT

14

N.S.

+

Leu2

TTA

7

-

N.S.

TTG

6

N.S.

N.S.

Leu4

CTA*

3

-

N.S.

CTC^W

0

-

-

CTG

10

+

N.S.

CTT

12

-

N.S.

Lys

AAA

17

-

N.S.

AAG

17

N.S.

N.S.

Phe

TTC

12

N.S.

N.S.

TTT^W

0

N.S.

+

Pro

CCA

7

+

+

CCC^W

0

-

-

CCG*

4

-

-

CCT

10

N.S.

+

Ser2

AGC

8

N.S.

N.S.

AGT^W

0

N.S.

+

Ser4

TCA

5

N.S.

+

TCC^W

0

-

-

TCG*

4

-

-

TCT

11

+

+

Thr

ACA

8

N.S.

+

ACC^W

0

N.S.

-

ACG*

6

-

-

ACT

10

N.S.

N.S.

Tyr

TAC

14

N.S.

-

TAT^W

1

N.S.

+

Val

GTA*

5

N.S.

+

GTC^W

0

N.S.

N.S.

GTG

16

-

-

GTT

11

N.S.

+