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Figure 6 | BMC Genomics

Figure 6

From: A high-throughput screen identifying sequence and promiscuity characteristics of the loxP spacer region in Cre-mediated recombination

Figure 6

Effect of nucleotide location and sequence within the spacer on recombination frequency. Effect on recombination rate of changing one base at each degenerate spacer position from the sequence of the most promiscuous spacer (i.e. changing the base at position 1 from G to C creates the spacer CGGTAGGC which causes a 88% drop in total recombination relative to that of the most promiscuous spacer GGGTAGGC).

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