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Figure 1 | BMC Genomics

Figure 1

From: Knock-out of SO1377 gene, which encodes the member of a conserved hypothetical bacterial protein family COG2268, results in alteration of iron metabolism, increased spontaneous mutation and hydrogen peroxide sensitivity in Shewanella oneidensis MR-1

Figure 1

Schematic presentation of generation of in-frame deletion mutant of SO1377 gene A. Generation of a deletion construct using asymmetric crossover PCR; B. Integration of the suicide plasmid during a single crossover event; C. Generation of in-frame deletion during the excision of the suicide plasmid. More detail about this construction process was described under "methods".

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