Figure 1From: Knock-out of SO1377 gene, which encodes the member of a conserved hypothetical bacterial protein family COG2268, results in alteration of iron metabolism, increased spontaneous mutation and hydrogen peroxide sensitivity in Shewanella oneidensis MR-1Schematic presentation of generation of in-frame deletion mutant of SO1377 gene A. Generation of a deletion construct using asymmetric crossover PCR; B. Integration of the suicide plasmid during a single crossover event; C. Generation of in-frame deletion during the excision of the suicide plasmid. More detail about this construction process was described under "methods".Back to article page