Figure 2

PCR verification of 7 potential preferentially expressed tags identified using the TPE algorithm. The expression of these seven genes was assessed both under resting conditions and after cytokine exposure in all cell types. On the right, the TPE values of the gene are listed for both PBEC and KC after cytokine exposure. Tags for which no TPE value could be calculated because of absence of the tag in the particular library are indicated by "not available" (N/A). The predicted preferential expression could be verified for all genes. The expression of six of these genes seems to be selective for epithelial cells only. Whereas SPRR1B is preferentially expressed by epithelial cells, moderate to low levels of expression were also detected in other cell types as well. SPRR2A is preferentially expressed by PBEC only. No tags for SPRR2A were found in the KC libraries after TNFα exposure whereas expression for CALML5 was observed by RT-PCR in both PBEC and KC, while no tags for this gene were found in PBEC libraries.