Histological staining methods preparatory to laser capture microdissection significantly affect the integrity of the cellular RNA

Table 1 Number of genes significantly differently expressed (p < 0.001 and > 2-fold mean expression difference) after LCM and staining.

Intensity Ratios	MGP/A	H&E/A	NS/A	IF/A
Total number	21	44	70	44
2-fold lower	13	29	40	31
2-fold higher	8	15	30	13
Net lower	5	14	10	18

Shown are numbers of genes with greater than two-fold change in expression when means of each group, e.g., MGP-stained, LCM-procured cells, were compared with the gene expression profile "A" from the pelleted, unstained sample (not LCM-procured). Net genes lower was calculated as total genes lower minus the number higher.

ISSN: 1471-2164