Histological staining methods preparatory to laser capture microdissection significantly affect the integrity of the cellular RNA

Table 2 Number of spots differently hybridizing after staining with stains other than MGP.

Intensity Ratios	H&E/MGP	NS/MGP	IF/MGP
Total number	61	147	112
2-fold lower	52	115	89
2-fold higher	9	32	23
Net lower	43	83	66

Shown are numbers of genes with greater than two-fold change in expression when means of each group, e.g., H&E-stained, LCM-procured cells, were compared with the gene expression profile of the MGP-stained, LCM-procured RNA. Net genes lower was calculated as total genes lower minus the number higher.

ISSN: 1471-2164