Figure 4
From: TAMGeS: a Three-Array Method for Genotyping of SNPs by a dual-colour approach
SNP genotyping by TAMGeS. On the upper left an image of our microarrays (tagged "array of arrays" format) on which 48 separate reaction chambers are created. On three distinct arrays (P 1 , P 2 and U), we hybridize the SBE reactions performed on each DNA sample with three different combinations of Cy3 or Cy5 labelled ddNTPs. The U-array is exploited to normalize (N) signals from arrays P 1 and P 2 , in order to compare them directly (dashed lines) and to genotype all the possible polymorphic alleles.