Table 1 Multiplex PCR primer pairs
From: TAMGeS: a Three-Array Method for Genotyping of SNPs by a dual-colour approach
SNPs | SEQUENCE (FORWARD and REVERSE) | μM | PRODUCT LENGTH |
|---|---|---|---|
rs3738701 | 5'- ACCTGAGAGGGCAAGTCAGAACCAACT -3' 5'- CGAGGTCCTGTGTTCGGCAACTTTA -3' | 0.16 0.16 | 197 |
rs11466112 | 5'- CAAAGCACTGGAACTCATATTGTACCACG -3' 5'- AATAATTTACAGGTTGAGGTAGGGAGGGG -3' | 0.32 0.32 | 221 |
X60202 | 5'- TAATGAGACACCCACCGCTGCTGTG -3' 5'- TCTACCGGAGGGGAGGAAAGAAGGAGAC -3' | 1.60 1.60 | 262 |
rs741072 rs734194 | 5'- GAAGCATCGGAGGGAATTGAGGTCTG -3' 5'- GCCAAGCTCAGGAAAAAACAGTCCTTTG -3' | 0.28 0.28 | 301 |
rs2289656 rs2289658 | 5'- TGCCTAACAAATGAGATGGATGTCTTTCCT -3' 5'- GAACCCTCCACTCCTGAACCCTGAA -3' | 0.24 0.40 | 325 |
rs6336 rs6339 rs1799770 | 5'- TGCCTCTACTGTTCTCTCAATCCTCCACTT -3' 5'- CCTGCTCATGCCAAAATCACCAATCTT -3' | 0.24 0.24 | 352 |
rs6265 rs3750934 rs8192466 | 5'- GGGGAAACACTGCATGTCTCTGGTTTATAT -3' 5'- GACTACTGAGCATCACCCTGGACGTGTAC -3' | 0.24 0.24 | 530 |
rs2275857 | 5'- AACCACCCTCCCTTCCTTTCTCTAGGTCTA -3' 5'- AACCCTTCTTACCTTTCATGCCAAACTTG -3' | 0.16 0.16 | 136 |
rs1047856 | 5'- GCTTCAGTGGTTCTATAACGGGGCAATAT -3' 5'- CTCATCCTTCCCATACTCATTCTTGGCTATT -3' | 0.32 0.32 | 171 |
rs3753213 | 5'- CACCGCCTAGTCCCTTGGTTCTGAC -3' 5'- GTGGCTGCACTAACCCATCCGTCTC -3' | 1.44 1.44 | 291 |
rs1800878 | 5'- GCATGTGCATGTGTATTGTGAGGGAGTAAG -3' 5'- CTCAAAGCCCTGAGCTTCCTGACTTCTC -3' | 0.32 0.32 | 328 |
rs2072446 rs2072445 | 5'- GCCAGAGTCACCCAGCAAGTCAGTG -3' 5'- CCGTGCTGGCTATGAGGTCTTGTTCT -3' | 0.16 0.16 | 347 |
rs6330 rs11466110 rs11466111 | 5'- TGCATAGCGTAATGTCCATGTTGTTCTACA -3' 5'- GATGATGACCGCTTGCTCCTGTGAGTC -3' | 0.32 0.32 | 382 |
rs1048218 rs1048220 rs1048221 | 5'- AAGAGGCTTGACATCATTGGCTGACACT -3' 5'- CATGGGATTGCACTTGGTCTCGTAGA -3' | 0.32 0.32 | 410 |