Figure 4

Scorpion toxin-like precursors in H. gertschi venom gland library. A) Predicted amino acid sequences of the potential α-KTx. HGE024|Contig2 predicted sequence is aligned with all members of the α-KTx 6 subfamily. HGE025|Contig5 is aligned with anuroctoxin (α-KTx 6.12). PSI-BLAST e-values for the third iteration are shown. B) Predicted amino acid sequence of Hg scorpine like 2 and its alignment with others members of the scorpine-like group. The percentage of identity with scorpine is shown. See Supplementary Figure 1 for the complete nucleotide sequences of HGE024|Contig2, HGE025|Contig5 and Hg scorpine like 2. Each sequence starts with its SwissProt accession number followed by common names and Protein Data Bank codes between parentheses (where available). Systematic numbering (sensu [47,49]) for α-KTx is included between accession numbers and common names. Identical amino acids are in red colour and conserved ones in green.