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Table 1 Summary of cDNA libraries and EST data

From: Comparative analysis of differentially expressed genes in normal and white spot syndrome virus infected Penaeus monodon

  Library
  PmTwN   PmTwI
Category Number of sequences
Total sequenced cDNA1 3':6964, 5':1036   3':7686, 5':1119
High quality ESTs1 3':6658, 5': 978   3':7276, 5':1069
cDNA clones that produced high quality ESTs 6671   7298
Mean EST length (bp) 678   652
Unique sequences   9622  
Mean unique sequence length (bp)   678  
Contigs   1364  
Matched unique sequence (nr db)   2027 (21.07%2)  
Matched unique sequence (UniProt db)   2026 (21.06%2)  
Matched ESTs3 (nr db) 3022 (45.30%4)   3338 (45.74%4)
Matched ESTs3 (UniProt db) 2870 (43.02%4)   3202 (43.88%4)
  1. 1: The numbers of 3' and 5' end sequences are shown.
  2. 2: The percentage is calculated by dividing the number of unique sequences (9622) by the number of matched unique sequences (2027 and 2026, respectively).
  3. 3: Some cDNA clones have both 5' and 3' ESTs, and when both or either ESTs had matches in databases, the corresponding 5' and 3' ESTs from the same clone will be marked and only one was counted.
  4. 4: The percentage is calculated by dividing the number of cDNA clones that produced high quality ESTs (6671 or 7298) by the number of matched ESTs (3022 and 3338, respectively, or 2870 and 3202).