Figure 6

Representative PCR/LDR data for the bapC HPT. Raw capillary electrophoresis data for ligation products from bapC multiplex PCR/LDR (green) and molecular weight standards (red) displayed as if an electrophoretic gel image. Strains and HPT allele lengths are indicated above each lane. Ligation products (arrows) are labeled using the notation described in the legend to Figure 1. Letters in teal indicate molecular weight standards as labeled in Additional file 18. (B) Representative traces from direct sequencing of bapC PCR products. Strain and alleles are noted above each sequence trace. Purple arrows indicate the point in the sequence trace after which peak shadowing, indicative of a mixture of HPT alleles, can be observed.