Figure 2
Analyses of the genomic structure of the Medaka fas, fadd and casp8 genes. (A) Genomic organization of the Medaka and human FAS (TNFRSF6) genes. Both Medaka and human FAS are composed of nine exons. The coding and non-coding regions are indicated by black and white boxes, respectively. The majority of the exons of the Medaka fas gene were identified in the genomic sequence from scaffold1622 (exon 1: 72, 135-72, 214), scaffold28293 (exons 3 and 4: 5, 721-5, 396; exons 7–9: 4, 964-2, 872) and scaffold100324 (exons 5 and 6: 405-121). (B) Comparison of the splice junction sites in Medaka, human and chicken Fas. Arrowheads on amino acid alignment indicate the splice junction sites in the Medaka, human and chicken genes, respectively. (C) Genomic organization of the Medaka and human FADD genes. Both the Medaka fadd and human FADD genes contain two exons. The black and white boxes correspond to the coding and non-coding regions, respectively. The fadd gene was detected from the genomic sequence (23, 960-15, 238) of scaffold7231. (D) Comparison of the splice junction sites of FADD from several vertebrates. An arrowhead indicates the splice junction site, defined by the comparison of the cDNA and the genomic sequences of human (H. sapiens), mouse (M. musculus), chicken(G. gallus), frog (X. tropicalis), zebrafish (D. rerio), stickleback (G. aculeatus) and Medaka (O. latipes). (E, F) Genomic organization of the Medaka and human CASP8 genes. The Medaka casp8 gene was identified in the genomic sequence (1, 102, 834-1, 096, 083) of scaffold169 (E). The Medaka casp8 gene is composed of 12 exons, while the human CASP8 gene is 9 exons. The coding and non-coding regions are indicated by black and white boxes, respectively. Comparison of the splice junction sites in Medaka, stickleback, zebrafish and human caspase-8 is shown in (F). Arrowheads on amino acid alignment indicate the splice junction sites in the Medaka, stickleback, zebrafish and human genes, respectively. Abbreviations: CRD, cysteine repeat domain; DD, death domain; DED, death effector domain; TM, transmembrane domain.