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Table 1 Putative genes idenitified in cDNA clones of Puccinia striiformis f. sp. tritici based on their sequence comparison with other fungal genes through Blastx search of the NCBI databases

From: Construction and characterization of a full-length cDNA library for the wheat stripe rust pathogen (Puccinia striiformis f. sp. tritici)

Category & clone no. GenBank accession Size (bp) Full length or partiala Best hit in the NCBI fungal databases
     Protein Accession Organism e-value
1. Amino acid metabolism
65N4 EG374380 2044 F Glycine hydroxymethyltransferase gb|AAW45780.1 Cryptococcus neoformans 1.00E-156
60J18a EG374421 1142 P Potential kynurenine 3-monooxygenase gb|EAK98864.1 Candida albicans 2.00E-06
60J18b EG374422 1220 P Potential kynurenine 3-monooxygenase gb|EAK98864.1 Candida albicans 1.00E-12
58D15a EG374299 897 P Saccharopine dehydrogenase gi|70993695 Aspergillus fumigatus 2.00E-55
58D15b EG374300 780 P Spermidine synthase emb|CAD71251.1 Neurospora crassa 3.00E-78
2. Cell Defense
35A16 EG374447 1351 F Related to stress response protein emb|CAD21425.1 Neurospora crassa 2.00E-23
3. Cell division/cycle
80F12 EG374389 1560 F Cell division control protein gb|AAB69764.1 Candida albicans 2.00E-28
65O23 EG374383 2037 F Cyclin c homolog 1 ref|NP_596149.1 Schizosaccharomyces pombe 3.00E-07
4. Cell signaling/cell communication
40D3 EG374466 1534 F Autophagy-related protein gb|AAW43831.1 Cryptococcus neoformans 6.00E-45
70C17a EG374441 1206 P Fasciclin I family protein gi|44890027 Aspergillus fumigatus 3.00E-06
58J15b EG374311 807 P GTPase activating protein gb|AAW43777.1 Cryptococcus neoformans 2.00E-09
55B10a EG374277 861 P MAP kinase 1 gb|AAO61669.1 Cryptococcus neoformans 3.00E-19
55B10b EG374278 932 P MAP kinase gb|AAU11317.1 Alternaria brassicicola 7.00E-74
65M20 EG374379 1098 F Nucleoside-diphosphate kinase emb|CAD37041.1 Neurospora crassa 9.00E-53
70E5 EG374404 1766 F Serine/threonine kinase gi|58262703 Cryptococcus neoformans 3.00E-61
10D13a EG374414 1122 P Serine palmitoyl transferase subunit gb|AAP47107.1 Aspergillus nidulans 4.00E-27
10D13b EG374416 1170 P Serine palmitoyl transferase subunit gb|AAP47107.1 Aspergillus nidulans 2.00E-18
30G12 EG374337 1131 F Signal peptidase 18 KD subunit emb|CAE76335.1 Neurospora crassa 3.00E-10
5. Cell Structure and growth
58H22a EG374306 920 P Beta-tubulin emb|CAC83953.1 Uromyces viciae-fabae 3.00E-72
58H22b EG374307 859 P Beta-tubulin emb|CAC83953.1 Uromyces viciae-fabae 5.00E-68
10I12 EG374325 1105 F Conidiation protein 6 emb|CAD70456.1 Neurospora crassa 2.00E-10
30J9 EG374343 1302 F Deacetylase emb|CAD10036.1 Cryptococcus neoformans 2.00E-43
60C15 EG374348 1456 F Deacetylase gb|AAW47023.1 Cryptococcus neoformans 6.00E-35
65D17 EG374372 1449 F Deacetylase emb|CAD10036.1 Cryptococcus neoformans 4.00E-36
40F18 EG374469 1117 F Deacetylase emb|CAD10036.1 Cryptococcus neoformans 2.00E-31
55D17 EG374475 1619 F Deacetylase emb|CAD10036.1 Cryptococcus neoformans 5.00E-18
35C19b EG374494 836 P Deacetylase emb|CAD10036.1 Cryptococcus neoformans 6.00E-18
10C3 EG374321 1479 F Deacetylase gb|AAW47023.1 Cryptococcus neoformans 6.00E-26
35N24 EG374461 783 F Hydrophobin emb|CAD42710.1 Davidiella tassiana 5.00E-34
32H21a EG374436 1176 P Intraorganellar peroxisomal translocation component Pay32p (PAY32) gene gi|5821763 Yarrowia lipolytica 4.00E-32
40B22 EG374465 1708 F Nuclear filament-containing protein emb|CAA93293.1| Schizosaccharomyces pombe 5.00E-16
35G11a EG374497 819 P Pria_lened pria protein emb|CAA43289.1 Lentinula edodes 2.00E-12
65M2 EG374413 2097 F UDP-glucose dehydrogenase gb|AAS20528.1 Cryptococcus neoformans 1.00E-145
6. Energy/TCA cycle
35D23b EG374496 629 P 64 kDa mitochondrial NADH dehydrogenase gb|AAW44492.1 Cryptococcus neoformans 1.00E-07
40H12 EG374471 1249 F Iron-sulfur cluster Isu1-like protein gb|AAQ98966.1 Cryptococcus neoformans 8.00E-56
55E23a EG374279 957 P Mitochondrial ATPase alpha-subunit gb|AAA33560.1 Neurospora crassa 6.00E-78
55E23b EG374280 870 P Mitochondrial ATPase alpha-subunit gb|AAA33560.1 Neurospora crassa 1.00E-101
90M15 EG374409 1570 F Mitochondrial carrier family protein gb|EAK95613.1 Candida albicans 1.00E-46
30N15a EG374419 1078 P Succinate dehydrogenase flavoprotein subunit precursor gb|AAW45324.1 Cryptococcus neoformans 1.00E-63
30N15b EG374420 1143 P Succinate dehydrogenase flavoprotein subunit precursor gb|AAW45324.1 Cryptococcus neoformans 1.00E-136
10A2 EG374481 1114 F V-type ATPase subunit G gb|AAB41886.1| Neurospora crassa 6.00E-15
7. Lipid metabolism
65D3 EG374370 1809 F Diacylglycerol O-acyltransferase gi|58268157 Cryptococcus neoformans 1.00E-84
65G21a EG374424 1078 P Fatty acid oxidoreductase gb|AAW46114.1 Cryptococcus neoformans 2.00E-05
65G21b EG374425 1149 P Fatty acid oxidoreductase gb|AAW46114.1 Cryptococcus neoformans 3.00E-32
58J11b EG374309 732 P Phosphatidyl synthase gi|70999337 Aspergillus fumigatus 2.00E-20
8. Nucleotide metabolism
58C19a EG374297 827 P Uracil DNA N-glycosylase gb|AAW41098.1 Cryptococcus neoformans 7.00E-16
58C19b EG374298 857 P Uracil DNA N-glycosylase gb|AAW41098.1 Cryptococcus neoformans 1.00E-19
9. Protein modification
65B1 EG374366 1847 F Carboxypeptidase gi|19115337 Schizosaccharomyces pombe 7.00E-06
66B11a EG374437 1145 P Endopeptidase gb|AAW41068.1 Cryptococcus neoformans 2.00E-69
66B11b EG374438 1200 P Endopeptidase gb|AAW41068.1 Cryptococcus neoformans 1.00E-48
80N15 EG374397 1944 F Translation elongation factor eEF-1 alpha chain pir||S57200 Puccinia graminis 0.00E+00
10. Protein translational modification
55N13 EG374483 833 F Ubiquitin-conjugating enzyme ref|NP_594859.1 Schizosaccharomyces pombe 7.00E-21
11. Ribosomal protein complex
55B4 EG374472 770 F 16S small subunit ribosomal RNA gi|52699765 Xanthoria elegans 2.00E-08
35O22 EG374462 938 F 18S ribosomal RNA gi|21702995 Gymnosporangium libocedri 1.00E-154
60E22 EG374352 1117 F 18S ribosomal RNA gi|34493860 Puccinia graminis f. sp.tritici 3.00E-142
65C12 EG374368 1136 F 18S ribosomal RNA gi|34493860 Puccinia graminis f. sp.tritici 2.00E-66
90D5a EG374432 1119 P 18S ribosomal RNA gi|21724233 Puccinia striiformis f. sp.tritici 6.00E-102
90D5b EG374431 1147 P ITS1, ITS2 and 5.8S ribosomal RNA gi|3668067 Tricholoma matsutake 9.00E-54
58E11b EG374302 831 P 25S ribosomal RNA gi|169606 Puccinia graminis f. sp. dactylis 1.00E-09
23H10b EG374283 1921 F 28S ribosomal RNA gi|37703614 Puccinia allii 1.00E-83
35M12a EG374458 763 F 28S ribosomal RNA gi|21724230 Puccinia graminis f. sp. tritici 2.00E-14
35N2 EG374460 917 F 28S ribosomal RNA gi|46810582 Fuscoporia viticola 4.00E-06
35P13 EG374463 888 F 28S ribosomal RNA gi|86160913 Melampsora epitea 2.00E-16
40A4 EG374464 951 F 28S ribosomal RNA gi|58532805 Puccinia carthami 4.00E-05
55J11 EG374479 957 F 28S ribosomal RNA gi|21724233 Puccinia striiformis f. sp. tritici 2.00E-26
35I10b EG374502 422 P 28S ribosomal RNA gi|21914221 Puccinia graminis 5.00E-77
35I22a EG374505 716 P 28S ribosomal RNA gi|21914221 Puccinia graminis 2.00E-70
35I22b EG374504 878 P ITS1, ITS2 and 5.8S ribosomal RNA gi|21724233 Puccinia striiformis f. sp.tritici 5.00E-134
10G18 EG374323 1108 F 28S ribosomal RNA gi|84452427 Cladosporium cladosporioides 1.00E-59
30C19 EG374333 1117 F 28S ribosomal RNA gi|62005831 Puccinia ferruginosa 2.00E-13
30H3 EG374340 1052 F 28S ribosomal RNA gi|21724233 Puccinia striiformis f. sp. tritici 3.00E-71
30I12 EG374341 1067 F 28S ribosomal RNA gi|21724233 Puccinia striiformis f. sp. tritici 2.00E-39
30M20 EG374347 1008 F 28S ribosomal RNA gi|21914221 Puccinia graminis 1.00E-93
60J23 EG374357 2112 F calnexin gb|AAS68033.1 Aspergillus fumigatus 1.00E-133
12. Sugar/glycolysis metabolism
30I15b EG374418 617 P Glucose-repressible protein emb|CAC28672.1 Neurospora crassa 2.00E-14
90C20 EG374401 1130 F Glucose-repressible protein gi|70996962 Aspergillus fumigatus 7.00E-18
55J22b EG374287 887 P Glyoxal oxidase precursor gb|AAW44259.1 Cryptococcus neoformans 2.00E-90
55J22a EG374286 764 P Glyoxal oxidase precursor gb|AAW41343.1 Cryptococcus neoformans 3.00E-30
90H16 EG374405 1753 F Phosphopyruvate hydratase gi|1086120 Cladosporium herbarum 1.00E-139
30K8 EG374344 1547 F Transaldolase gb|AAW46393.1 Cryptococcus neoformans 3.00E-95
13. Transcription factor
58E6 EG374485 1310 F TATA-box binding protein gb|AAB57876.1 Emericella nidulans 7.00E-63
14. Transport metabolism
65M6 EG374378 1119 F Cation transport-related protein gb|AAW42114.1 Cryptococcus neoformans 3.00E-13
15. virulence/infection related protein
70I2 EG374433 1952 F Cell wall glucanase gi|70998053 Aspergillus fumigatus 2.00E-25
30M9 EG374345 1162 F Differentiation-related/infection protein gb|AAD38996.1 Uromyces appendiculatus 7.00E-11
80C7 EG374385 1180 F Differentiation-related/infection protein gb|AAD38996.1 Uromyces appendiculatus 1.00E-10
60E18 EG374351 2147 F Pectin lyase gb|AAA21817.1 Glomerella cingulata 2.00E-06
  1. a F = full-length sequence and P = partial sequence.