Construction and characterization of a full-length cDNA library for the wheat stripe rust pathogen (Puccinia striiformis f. sp. tritici)

Ling, Peng; Wang, Meinan; Chen, Xianming; Campbell, Kimberly Garland

doi:10.1186/1471-2164-8-145

Table 1 Putative genes idenitified in cDNA clones of Puccinia striiformis f. sp. tritici based on their sequence comparison with other fungal genes through Blastx search of the NCBI databases

From: Construction and characterization of a full-length cDNA library for the wheat stripe rust pathogen (Puccinia striiformis f. sp. tritici)

Category & clone no.	GenBank accession	Size (bp)	Full length or partial^a	Best hit in the NCBI fungal databases
				Protein	Accession	Organism	e-value
1. Amino acid metabolism
65N4	EG374380	2044	F	Glycine hydroxymethyltransferase	gb\|AAW45780.1	Cryptococcus neoformans	1.00E-156
60J18a	EG374421	1142	P	Potential kynurenine 3-monooxygenase	gb\|EAK98864.1	Candida albicans	2.00E-06
60J18b	EG374422	1220	P	Potential kynurenine 3-monooxygenase	gb\|EAK98864.1	Candida albicans	1.00E-12
58D15a	EG374299	897	P	Saccharopine dehydrogenase	gi\|70993695	Aspergillus fumigatus	2.00E-55
58D15b	EG374300	780	P	Spermidine synthase	emb\|CAD71251.1	Neurospora crassa	3.00E-78
2. Cell Defense
35A16	EG374447	1351	F	Related to stress response protein	emb\|CAD21425.1	Neurospora crassa	2.00E-23
3. Cell division/cycle
80F12	EG374389	1560	F	Cell division control protein	gb\|AAB69764.1	Candida albicans	2.00E-28
65O23	EG374383	2037	F	Cyclin c homolog 1	ref\|NP_596149.1	Schizosaccharomyces pombe	3.00E-07
4. Cell signaling/cell communication
40D3	EG374466	1534	F	Autophagy-related protein	gb\|AAW43831.1	Cryptococcus neoformans	6.00E-45
70C17a	EG374441	1206	P	Fasciclin I family protein	gi\|44890027	Aspergillus fumigatus	3.00E-06
58J15b	EG374311	807	P	GTPase activating protein	gb\|AAW43777.1	Cryptococcus neoformans	2.00E-09
55B10a	EG374277	861	P	MAP kinase 1	gb\|AAO61669.1	Cryptococcus neoformans	3.00E-19
55B10b	EG374278	932	P	MAP kinase	gb\|AAU11317.1	Alternaria brassicicola	7.00E-74
65M20	EG374379	1098	F	Nucleoside-diphosphate kinase	emb\|CAD37041.1	Neurospora crassa	9.00E-53
70E5	EG374404	1766	F	Serine/threonine kinase	gi\|58262703	Cryptococcus neoformans	3.00E-61
10D13a	EG374414	1122	P	Serine palmitoyl transferase subunit	gb\|AAP47107.1	Aspergillus nidulans	4.00E-27
10D13b	EG374416	1170	P	Serine palmitoyl transferase subunit	gb\|AAP47107.1	Aspergillus nidulans	2.00E-18
30G12	EG374337	1131	F	Signal peptidase 18 KD subunit	emb\|CAE76335.1	Neurospora crassa	3.00E-10
5. Cell Structure and growth
58H22a	EG374306	920	P	Beta-tubulin	emb\|CAC83953.1	Uromyces viciae-fabae	3.00E-72
58H22b	EG374307	859	P	Beta-tubulin	emb\|CAC83953.1	Uromyces viciae-fabae	5.00E-68
10I12	EG374325	1105	F	Conidiation protein 6	emb\|CAD70456.1	Neurospora crassa	2.00E-10
30J9	EG374343	1302	F	Deacetylase	emb\|CAD10036.1	Cryptococcus neoformans	2.00E-43
60C15	EG374348	1456	F	Deacetylase	gb\|AAW47023.1	Cryptococcus neoformans	6.00E-35
65D17	EG374372	1449	F	Deacetylase	emb\|CAD10036.1	Cryptococcus neoformans	4.00E-36
40F18	EG374469	1117	F	Deacetylase	emb\|CAD10036.1	Cryptococcus neoformans	2.00E-31
55D17	EG374475	1619	F	Deacetylase	emb\|CAD10036.1	Cryptococcus neoformans	5.00E-18
35C19b	EG374494	836	P	Deacetylase	emb\|CAD10036.1	Cryptococcus neoformans	6.00E-18
10C3	EG374321	1479	F	Deacetylase	gb\|AAW47023.1	Cryptococcus neoformans	6.00E-26
35N24	EG374461	783	F	Hydrophobin	emb\|CAD42710.1	Davidiella tassiana	5.00E-34
32H21a	EG374436	1176	P	Intraorganellar peroxisomal translocation component Pay32p (PAY32) gene	gi\|5821763	Yarrowia lipolytica	4.00E-32
40B22	EG374465	1708	F	Nuclear filament-containing protein	emb\|CAA93293.1\|	Schizosaccharomyces pombe	5.00E-16
35G11a	EG374497	819	P	Pria_lened pria protein	emb\|CAA43289.1	Lentinula edodes	2.00E-12
65M2	EG374413	2097	F	UDP-glucose dehydrogenase	gb\|AAS20528.1	Cryptococcus neoformans	1.00E-145
6. Energy/TCA cycle
35D23b	EG374496	629	P	64 kDa mitochondrial NADH dehydrogenase	gb\|AAW44492.1	Cryptococcus neoformans	1.00E-07
40H12	EG374471	1249	F	Iron-sulfur cluster Isu1-like protein	gb\|AAQ98966.1	Cryptococcus neoformans	8.00E-56
55E23a	EG374279	957	P	Mitochondrial ATPase alpha-subunit	gb\|AAA33560.1	Neurospora crassa	6.00E-78
55E23b	EG374280	870	P	Mitochondrial ATPase alpha-subunit	gb\|AAA33560.1	Neurospora crassa	1.00E-101
90M15	EG374409	1570	F	Mitochondrial carrier family protein	gb\|EAK95613.1	Candida albicans	1.00E-46
30N15a	EG374419	1078	P	Succinate dehydrogenase flavoprotein subunit precursor	gb\|AAW45324.1	Cryptococcus neoformans	1.00E-63
30N15b	EG374420	1143	P	Succinate dehydrogenase flavoprotein subunit precursor	gb\|AAW45324.1	Cryptococcus neoformans	1.00E-136
10A2	EG374481	1114	F	V-type ATPase subunit G	gb\|AAB41886.1\|	Neurospora crassa	6.00E-15
7. Lipid metabolism
65D3	EG374370	1809	F	Diacylglycerol O-acyltransferase	gi\|58268157	Cryptococcus neoformans	1.00E-84
65G21a	EG374424	1078	P	Fatty acid oxidoreductase	gb\|AAW46114.1	Cryptococcus neoformans	2.00E-05
65G21b	EG374425	1149	P	Fatty acid oxidoreductase	gb\|AAW46114.1	Cryptococcus neoformans	3.00E-32
58J11b	EG374309	732	P	Phosphatidyl synthase	gi\|70999337	Aspergillus fumigatus	2.00E-20
8. Nucleotide metabolism
58C19a	EG374297	827	P	Uracil DNA N-glycosylase	gb\|AAW41098.1	Cryptococcus neoformans	7.00E-16
58C19b	EG374298	857	P	Uracil DNA N-glycosylase	gb\|AAW41098.1	Cryptococcus neoformans	1.00E-19
9. Protein modification
65B1	EG374366	1847	F	Carboxypeptidase	gi\|19115337	Schizosaccharomyces pombe	7.00E-06
66B11a	EG374437	1145	P	Endopeptidase	gb\|AAW41068.1	Cryptococcus neoformans	2.00E-69
66B11b	EG374438	1200	P	Endopeptidase	gb\|AAW41068.1	Cryptococcus neoformans	1.00E-48
80N15	EG374397	1944	F	Translation elongation factor eEF-1 alpha chain	pir\|\|S57200	Puccinia graminis	0.00E+00
10. Protein translational modification
55N13	EG374483	833	F	Ubiquitin-conjugating enzyme	ref\|NP_594859.1	Schizosaccharomyces pombe	7.00E-21
11. Ribosomal protein complex
55B4	EG374472	770	F	16S small subunit ribosomal RNA	gi\|52699765	Xanthoria elegans	2.00E-08
35O22	EG374462	938	F	18S ribosomal RNA	gi\|21702995	Gymnosporangium libocedri	1.00E-154
60E22	EG374352	1117	F	18S ribosomal RNA	gi\|34493860	Puccinia graminis f. sp.tritici	3.00E-142
65C12	EG374368	1136	F	18S ribosomal RNA	gi\|34493860	Puccinia graminis f. sp.tritici	2.00E-66
90D5a	EG374432	1119	P	18S ribosomal RNA	gi\|21724233	Puccinia striiformis f. sp.tritici	6.00E-102
90D5b	EG374431	1147	P	ITS1, ITS2 and 5.8S ribosomal RNA	gi\|3668067	Tricholoma matsutake	9.00E-54
58E11b	EG374302	831	P	25S ribosomal RNA	gi\|169606	Puccinia graminis f. sp. dactylis	1.00E-09
23H10b	EG374283	1921	F	28S ribosomal RNA	gi\|37703614	Puccinia allii	1.00E-83
35M12a	EG374458	763	F	28S ribosomal RNA	gi\|21724230	Puccinia graminis f. sp. tritici	2.00E-14
35N2	EG374460	917	F	28S ribosomal RNA	gi\|46810582	Fuscoporia viticola	4.00E-06
35P13	EG374463	888	F	28S ribosomal RNA	gi\|86160913	Melampsora epitea	2.00E-16
40A4	EG374464	951	F	28S ribosomal RNA	gi\|58532805	Puccinia carthami	4.00E-05
55J11	EG374479	957	F	28S ribosomal RNA	gi\|21724233	Puccinia striiformis f. sp. tritici	2.00E-26
35I10b	EG374502	422	P	28S ribosomal RNA	gi\|21914221	Puccinia graminis	5.00E-77
35I22a	EG374505	716	P	28S ribosomal RNA	gi\|21914221	Puccinia graminis	2.00E-70
35I22b	EG374504	878	P	ITS1, ITS2 and 5.8S ribosomal RNA	gi\|21724233	Puccinia striiformis f. sp.tritici	5.00E-134
10G18	EG374323	1108	F	28S ribosomal RNA	gi\|84452427	Cladosporium cladosporioides	1.00E-59
30C19	EG374333	1117	F	28S ribosomal RNA	gi\|62005831	Puccinia ferruginosa	2.00E-13
30H3	EG374340	1052	F	28S ribosomal RNA	gi\|21724233	Puccinia striiformis f. sp. tritici	3.00E-71
30I12	EG374341	1067	F	28S ribosomal RNA	gi\|21724233	Puccinia striiformis f. sp. tritici	2.00E-39
30M20	EG374347	1008	F	28S ribosomal RNA	gi\|21914221	Puccinia graminis	1.00E-93
60J23	EG374357	2112	F	calnexin	gb\|AAS68033.1	Aspergillus fumigatus	1.00E-133
12. Sugar/glycolysis metabolism
30I15b	EG374418	617	P	Glucose-repressible protein	emb\|CAC28672.1	Neurospora crassa	2.00E-14
90C20	EG374401	1130	F	Glucose-repressible protein	gi\|70996962	Aspergillus fumigatus	7.00E-18
55J22b	EG374287	887	P	Glyoxal oxidase precursor	gb\|AAW44259.1	Cryptococcus neoformans	2.00E-90
55J22a	EG374286	764	P	Glyoxal oxidase precursor	gb\|AAW41343.1	Cryptococcus neoformans	3.00E-30
90H16	EG374405	1753	F	Phosphopyruvate hydratase	gi\|1086120	Cladosporium herbarum	1.00E-139
30K8	EG374344	1547	F	Transaldolase	gb\|AAW46393.1	Cryptococcus neoformans	3.00E-95
13. Transcription factor
58E6	EG374485	1310	F	TATA-box binding protein	gb\|AAB57876.1	Emericella nidulans	7.00E-63
14. Transport metabolism
65M6	EG374378	1119	F	Cation transport-related protein	gb\|AAW42114.1	Cryptococcus neoformans	3.00E-13
15. virulence/infection related protein
70I2	EG374433	1952	F	Cell wall glucanase	gi\|70998053	Aspergillus fumigatus	2.00E-25
30M9	EG374345	1162	F	Differentiation-related/infection protein	gb\|AAD38996.1	Uromyces appendiculatus	7.00E-11
80C7	EG374385	1180	F	Differentiation-related/infection protein	gb\|AAD38996.1	Uromyces appendiculatus	1.00E-10
60E18	EG374351	2147	F	Pectin lyase	gb\|AAA21817.1	Glomerella cingulata	2.00E-06

^a F = full-length sequence and P = partial sequence.

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