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Table 2 cDNA clones showing homology to genes with characterized or unclassified proteins through Blastx search of the NCBI fungal databases

From: Construction and characterization of a full-length cDNA library for the wheat stripe rust pathogen (Puccinia striiformis f. sp. tritici)

Category & clone no. GenBank accession Size (bp) Full length or partiala Best hit in the NCBI databases
     Protein Accession Organism e-value
1. Amino acid metabolism
35I14 EG374455 766 F Cystathionine beta-lyase gi|6636350 Botryotinia fuckeliana 5.70E+00
2. Cell Defense
66C24a EG374440 1175 P 88 kDa immunoreactive mannoprotein MP88 gb|AAL87197.1 Cryptococcus neoformans 1.00E-03
3. Cell Division/cycle
10F19 EG374412 1877 F g1/s-specific cyclin pcl1 (cyclin hcs26) gb|AAW44590.1 Cryptococcus neoformans 2.00E-04
4. Cell signaling/cell communication
65G15 EG374514 1106 P Protein kinase gi|15072451 Cryphonectria parasitica 1.20E+00
30E21 EG374336 1128 F Serine/threonine kinase gi|22531808 Ustilago maydis 3.90E-01
65C6 EG374367 1649 F Serine/threonine phosphatase gi|33087517 Hypocrea jecorina 3.90E-01
80G5b EG374428 1230 P Mitogen-activated protein kinase gi|57227328 Cryptococcus neoformans 1.70E-00
5. Cell Structure and growth
58G9 EG374486 1714 F Beta tubulin gi|47834278 Penicillium flavigenum 6.40E-00
40G6b EG374274 888 P Cell wall protein gi|68471254 Candida albicans 4.60E-01
58C4b EG374296 819 P Cell surface protein gi|70983232 Aspergillus fumigatus 2.60E-02
10D19 EG374322 1212 F Cell wall mannoprotein ref|NP_012685.1 Saccharomyces cerevisiae 1.00E-03
90I19 EG374406 1240 F Cell wall mannoprotein gi|6322611 Saccharomyces cerevisiae 1.50E-02
90C22 EG374402 1641 F Cytoplasm protein gb|AAW42379.1 Cryptococcus neoformans 1.00E-04
10I15 EG374326 1088 F Mitochondrial outer membrane beta-barrel protein gi|45758780 Neurospora crassa 1.70E-01
60H1 EG374354 1035 F Nuclear pore complex subunit gi|46437749 Candida albicans 5.00E-00
70I19a EG374443 1132 P Nucleoskeletal-like protein gi|172053 Saccharomyces cerevisiae 1.30E-01
6. Differentiation- related protein
70A18 EG374371 1207 F Differentiation-related protein gb|AAD38996.1 Uromyces appendiculatus 6.00E-03
7. Mating type
30M10 EG374346 1025 F Mating type alpha locus gi|73914085 Cryptococcus gattii 6.80E+00
30C22 EG374334 1110 F Mating type alpha locus gi|73914085 Cryptococcus gattii 7.50E+00
8. Nucleotide metabolism
35K8 EG374456 1572 F Ribonuclease H2 subunit gi|6320485 Saccharomyces cerevisiae 9.00E+00
9. Protein translational modification
100C10 EG374490 1179 F Non-ribosomal peptide synthetase gi|62006079 Hypocrea virens 1.20E+00
10. Ribosomal protein complex
35L17 EG374457 585 F 18S ribosomal RNA gi|51102377 Microbotryum dianthorum 4.20E-02
40C19a EG374512 706 P 18S ribosomal RNA gi|28412377 Leotiomycete sp. 5.40E-01
35H2b EG374500 786 P 26S large subunit ribosomal RNA gi|30313824 Pichia guilliermondii AjvM13 1.00E-03
35E4 EG374451 897 F 28S ribosomal RNA gi|46810582 Fuscoporia viticola 5.00E-03
35P11a EG374506 667 P 28S ribosomal RNA gi|62005826 Puccinia artemisiae-keiskeanae 1.00E-04
55B15 EG374473 954 F 28S ribosomal RNA gi|84794517 Puccinia striiformoides 3.60E-01
58B3 EG374484 884 F 28S ribosomal RNA gi|46810582 Fuscoporia viticola 3.30E-01
58N22 EG374488 996 F 28S ribosomal RNA gi|20452324 Rhodotorula pilati 3.30E-01
66I12 EG374338 1167 F 28S ribosomal RNA gi|46810582 Fuscoporia viticola 3.00E-04
80G5a EG374427 1106 P Calnexin gi|45551624 Aspergillus fumigatus 2.30E-00
11. Sugar/glycolysis metabolism
58G18b EG374304 796 P Pyruvate decarboxylase gi|68480982 Candida albicans 1.40E+00
10N6 EG374330 1029 F Pyruvate kinase gi|168073 Aspergillus nidulans 6.00E+00
12. Transport metabolism
30G15 EG374339 1087 F Membrane zinc transporter gi|47156070 Aspergillus fumigatus 5.70E-01
40H8a EG374275 656 P amino acid transporter gi|70985369 Aspergillus fumigatus 3.10E+00
80K19 EG374395 1728 F Na+-ATPase gi|1777377 Zygosaccharomyces rouxii 2.00E-04
55L18b EG374289 845 P Peptide transporter gi|70982509 Aspergillus fumigatus 5.30E-01
13. Unclassified
80G10 EG374391 1132 F Genomic sequence gi|48056381 Phakopsora pachyrhizi 7.00E-53
04F9 EG374470 1127 F Hypothetical protein gi|71006713 Ustilago maydis 1.00E-06
10N10 EG374331 1106 F Hypothetical protein gi|58258450 Cryptococcus neoformans 6.00E-22
30I21 EG374342 1906 F Hypothetical protein gi|71023234 Ustilago maydis 1.00E-21
35B6 EG374449 1060 F Hypothetical protein gb|EAA67250.1 Gibberella zeae 1.00E-03
35C10 EG374450 1465 F Hypothetical protein gi|71004383 Ustilago maydis 521 2.00E-08
35G21 EG374454 1332 F Hypothetical protein gb|EAK81105.1 Ustilago maydis 5.00E-09
35H2a EG374499 758 P Hypothetical protein gi|71021872 Ustilago maydis 1.80E+00
40B2a EG374508 603 P Hypothetical protein gi|85114517 Neurospora crassa 3.00E-05
40C12a EG374510 792 P Hypothetical protein gi|71019552 Ustilago maydis 4.00E-01
55L8 EG374491 1417 F Hypothetical protein gi|71004813 Ustilago maydis 1.50E-01
58C4a EG374296 764 P Hypothetical protein MGG_09875.5b Magnaporthe grisea 6.00E-12
60D4 EG374350 1123 F Hypothetical protein gi|50259357 Cryptococcus neoformans 7.00E-04
60I14 EG374356 1565 F Hypothetical protein gi|58263159 Cryptococcus neoformans 2.00E-09
60L15 EG374359 2073 F Hypothetical protein gb|EAA47832.1 Magnaporthe grisea 7.00E-10
60N2 EG374363 1109 F Hypothetical protein gi|46096746 Ustilago maydis 7.00E-03
60N6 EG374364 1071 F Hypothetical protein gi|49642978 Kluyveromyces lactis 8.00E-17
65H5 EG374374 1390 F Hypothetical protein gi|85095053 Neurospora crassa 1.40E+00
65I3 EG374375 1870 F Hypothetical protein gb|EAK86140.1 Ustilago maydis 1.00E-129
65O15 EG374381 1893 F Hypothetical protein gi|71006255 Ustilago maydis 1.10E+00
66B6 EG374316 1263 F Hypothetical protein gb|EAK81690.1 Ustilago maydis 1.00E-03
66B11a EG374437 1145 P Hypothetical protein AN2903.3b Aspergillus nidulans 3.00E-57
66B11b EG374438 1200 P Hypothetical protein FG10782.1b Fusarium graminearum 5.00E-49
66C18 EG374327 2043 F Hypothetical protein gb|EAA59593.1 Aspergillus nidulans 2.00E-12
70A3 EG374360 1835 F Hypothetical protein SS1G_14513.1b Sclerotinia sclerotiorum 8.00E-18
70C17b EG374442 1191 P Hypothetical protein AN0768.3b Aspergillus nidulans 1.00E-07
70H16 EG374426 1121 F Hypothetical protein gi|38100779 Magnaporthe grisea 2.60E+00
70I19b EG374443 1190 P Hypothetical protein NCU02808.2b Neurospora crassa 2.00E-08
70K15b EG374320 933 P Hypothetical protein gi|58261561 Cryptococcus neoformans 1.00E-07
70L24b EG374446 1168 P Hypothetical protein gb|EAA28928.1 Neurospora crassa 3.00E-23
80I9 EG374394 1060 F Hypothetical protein gi|58259618 Cryptococcus neoformans 1.50E+00
90O3 EG374410 1725 F Hypothetical protein gi|85119288 Neurospora crassa 1.20E-02
90O18 EG374411 1973 F Hypothetical protein CHG04543.1b Chaetomium globosum 4.00E-07
66C24b EG374440 1271 P Macrofage activating glycoprotein gi|15722495 Cryptococcus neoformans 3.00E-08
30E3 EG374335 1406 F Probable gEgh 16 protein emb|CAE85538.1 Neurospora crassa 8.00E-07
60I8 EG374355 1039 F Related to ars binding protein 2 gi|18376044 Neurospora crassa 6.60E+00
55J15b EG374285 896 P Telomeric sequence DNA gi|173051 Saccharomyces cerevisiae 2.00E-05
55E7 EG374477 1253 F Unknown protein in chromosome E gi|49654999 Debaryomyces hansenii 3.00E-06
55F15a EG374281 461 P Unknown protein in chromosome G gi|50427978 Debaryomyces hansenii 2.00E-03
60L20 EG374361 1646 F Unknown protein in chromosome VI gi|39975020 Magnaporthe grisea 3.00E-18
60N1 EG374362 2024 F Unknown protein in chromosome 1 gi|46110618 Gibberella zeae 2.00E-09
70F20 EG374415 1818 F Unknown protein in chromosome III gi|58270250 Magnaporthe grisea 1.60E+00
80M4 EG374396 1985 F Unknown protein in chromosome G gi|49657202 Debaryomyces hansenii 1.00E-03
80N10 EG374430 563 P Phytochrome gi|57337632 Emericella nidulans 4.30E-00
90B8 EG374400 2011 F Unknown protein in chromosome G gi|49657202 Debaryomyces hansenii 4.90E-02
90L21 EG374408 2002 F Unknown protein in chromosome A gi|49524079 Candida glabrata 1.20E+00
  1. a F = full-length sequence and P = partial sequence.
  2. b Data generated from Blastx search of the fungal database of the Broad Institute [34].