Comparison of hybridization-based and sequencing-based gene expression technologies on biological replicates

BMC Genomics

Table 3 Summary of two-way ANOVA results

	RS	RSEXON
total # of genes (i.e. RSs or RSEXONs)	563	304
# of genes that showed neither sample- nor platform- related variation	293	170
# of genes that showed sample-related variation	27	13
# of genes that showed platform-related variation	260	132
# of genes that showed both sample- and platform- related variation	20	11

Two-way ANOVA was conducted for all microarray platforms that had been analyzed with technical replicates (Affymetrix, Amersham, Mergen, ABI). The genes that have valid measurements after normalization and filtering procedure in all technical replicates were included for the analysis. The analysis was conducted on gene expression measurements matched across platforms based on RefSeq ID (RS) and RefSeq Exon (RSEXON). A gene was said to have sample-related variation if the p-value of the sample effect size was significant (p < 0.001), and similarly for platform-related variation.

ISSN: 1471-2164