Figure 1

GeneChip results were confirmed for some genes using Northern blotting and RT-PCR. Examples are provided for both Northern blots (A) and RT-PCR (B). Note that the RT-PCR for the hacA mRNA was designed to indicate enhanced splicing of the hacA mRNA intron under stress conditions (DTT, tunicamycin and production of t-PA). This is shown as a relative increase in the amount of the processed (lower band) hacAⁱform of the mRNA compared to the unprocessed higher band (hacA^u). Probing or PCR for an actin gene was used as a non-stress-responsive control transcript.