Table 3 Examples of genes for which DTT treatment resulted in > 2 fold shift of transcripts from the non-polysomal to polysomal samples, or vice versa, relative to untreated controls.
DSM code | Predicted protein | Polysomal/non-polysomal Control | Polysomal/non-polysomal DTT treated | DTT induced shift |
|---|---|---|---|---|
From non-polysomal to polysomal fractions | ||||
An09g05420 | similar to signal peptidase subunit SPC3 | 4.61 | 0.64 | 7.20 |
An15g01130 | similar to brefeldin A resistance protein BFR1 (S. pombe) | 0.79 | 0,26 | 3.04 |
An01g14260 | similar to delta subunit of the coatomer delta-coat protein COPD (Bos. taurus) | 1.79 | 0.68 | 2.63 |
An17g00270 | similar to 26S ATP/ubiquitin-dependent proteinase chain S4 (S. pombe) | 3.13 | 1.28 | 2.45 |
From polysomal to non-polysomal fractions | ||||
Translation | ||||
An02g13840 | similar to cytoplasmic ribosomal protein of the small subunit S9 (H. sapiens) | 0.47 | 27.28 | 57.91 |
An18g05810 | similarity to cytoplasmic ribosomal protein of the small subunit S26 (H. sapiens) | 0.51 | 11.22 | 22,03 |
An08g00970 | ribosomal protein of the small subunit rps28 | 0.71 | 3.48 | 4.91 |
An11g09570 | similar to cytoplasmic ribosomal protein of the large subunit L37.b | 0.57 | 8.33 | 14.62 |
An12g04860 | similar to cytoplasmic ribosomal protein of the large subunit L30 | 0.37 | 4.18 | 11.42 |
An01g02880 | similar to cytoplasmic ubiquitin/ribosomal fusion protein CEP52 | 0.67 | 4.61 | 6.92 |
An18g04570 | similar to cleavage/polyadenylation factor IA subunit CLP1 | 0.71 | 1.56 | 2.20 |
An02g09260 | similar to nucleolar protein NOP5 | 2.12 | 9.22 | 4.34 |
An18g04840 | similar to elongation factor 1 alpha (Podospora anserina) | 1.88 | 6.02 | 3.21 |
An15g00750 | Similar to elongation factor 1-gamma 1 TEF3 | 2.16 | 5.35 | 2.47 |
An07g02650 | similar to translation elongation factor 3 YE3 | 2.96 | 23.88 | 8.07 |
Proteolytic degradation | ||||
An11g01760 | similar to proteasome 20S subunit PRE2 | 3.79 | 20.52 | 5.42 |
An02g07010 | similar to ubiquitin UBI1 (A. nidulans) | 1.1 | 7.5 | 7.12 |
Lipid metabolism | ||||
An08g10110 | similar to lipid transfer protein POX18 (C. tropicalis) | 0.96 | 4.04 | 4.23 |
An03g06410 | similar to methyl sterol oxidase ERG25 | 1.23 | 2.84 | 2.32 |
An12g01890 | similar to squalene synthase ERG9 (C. utilis) | 1.01 | 2.21 | 2.18 |
An02g10350 | similar to farnesyl-pyrophosphate synthase FPPS | 1.90 | 4.13 | 2.17 |
An07g09570 | similar to phosphatidate cytidylyltransferase CDS1 | 0.84 | 1.73 | 2.06 |
Cell Wall | ||||
An03g02400 | similar to hydrophobin DEWA (A. nidulans) | 0.51 | 1.59 | 3.10 |
An06g01550 | similar to glucan synthase FKS (Paracoccidioides brasiliensis) | 0.46 | 1.33 | 2.89 |
An11g00270 | similar to lanosterol 14 alpha-demethylase CYP51 (H. sapiens) | 0.64 | 1.44 | 2.25 |
An02g02360 | similar to chitin synthase (A. nidulans) | 0.43 | 0.96 | 2.24 |
Secreted proteins | ||||
An12g01910 | similar to phytase PHYA3 (A. fumigatus) | 0.36 | 4.03 | 11.1 |
An03g06550 | Glucoamylase GLAA | 0.57 | 17.69 | 30.98 |
An02g13220 | similar to lysophospholipase phospholipase B (P. notatum) | 0.55 | 2.90 | 5.23 |
An09g00270 | alpha-galactosidase AGLC | 0.38 | 1.65 | 4.3 |
An02g01550 | similar to serine protease (Coccidioides immitis) | 1.78 | 6.69 | 3.76 |
An02g04690 | similar to serine-type carboxypeptidase I CDPS (A. saitoi) | 1.71 | 3.56 | 2.08 |