Down-regulation of metabolic pathways by targeted knockdown of FAS. The figure displays select pathways found to be down-regulated in response to FAS siRNA treatment compared to non-silencing control siRNA. Significance was determined using a nominal p-value < 0.05 or FDR < 0.250. The expression levels of the genes significantly modified in the pathway are coded colorimetrically: red, high expression; blue, low expression. FAS siRNA treatments for each time point are ordered as follows (a and b indicate different biological replicates): FAS #1a, FAS #1b, FAS #2a, FAS #2b, FAS #3a, FAS #3b, FAS #4a, FAS #4b. For a complete list of all pathways down-regulated by knockdown of FAS, see Additional File 6.