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Table 2 Reproducibility of replicate spots within array

From: Normalization of low-density microarray using external spike-in controls: analysis of macrophage cell lines expression profile

Spike SDa) CVb)
sp 1 0.08 5.15%
sp 2 0.05 5.57%
sp 3 0.08 6.91%
sp 4 0.07 6.40%
sp 5 0.11 6.25%
sp 6 0.06 5.50%
sp 7 0.07 7.13%
sp 8 0.08 8.10%
  1. a)Standard deviation (SD) of M-values (M = log2R-log2G) was calculated for each series of 48 replicate spots within array. The averages of the SD of seven independent microarray experiments are shown. b) Coefficient of variation (CV) [(SD of M-values × 100)/mean of M-values] was calculated on the same sets of data and the averages of the seven independent microarray experiments are shown.
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