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Table 2 Reproducibility of replicate spots within array

From: Normalization of low-density microarray using external spike-in controls: analysis of macrophage cell lines expression profile

Spike

SDa)

CVb)

sp 1

0.08

5.15%

sp 2

0.05

5.57%

sp 3

0.08

6.91%

sp 4

0.07

6.40%

sp 5

0.11

6.25%

sp 6

0.06

5.50%

sp 7

0.07

7.13%

sp 8

0.08

8.10%

  1. a)Standard deviation (SD) of M-values (M = log2R-log2G) was calculated for each series of 48 replicate spots within array. The averages of the SD of seven independent microarray experiments are shown. b) Coefficient of variation (CV) [(SD of M-values × 100)/mean of M-values] was calculated on the same sets of data and the averages of the seven independent microarray experiments are shown.
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BMC Genomics

ISSN: 1471-2164

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