A global view of Staphylococcus aureus whole genome expression upon internalization in human epithelial cells

BMC Genomics

Table 2 List and characteristics of oligonucleotides used in the qPCR control experiments

Primer or Probe name	Sequence (5'→3')	Length	^A5'Dye	ORF number in N315
HU				SA1305
F_HU_ 34	CAAAGAGAAAACATGGTTACCATTATTAA	29
R_HU _135	CTCAAGCACCTCATAAGGATTATCAG	26
P_HU _83^A	AAAAGCCTATGGAAATTGCCCTCGCA	26	FAM
*agrA*				SA1844
F_agrA_ 34	CAAAGAGAAAACATGGTTACCATTATTAA	29
R_agrA _135	CTCAAGCACCTCATAAGGATTATCAG	26
P_agrA _83^A	AAAAGCCTATGGAAATTGCCCTCGCA	26	FAM
*sarS*				SA0108
F_sarS_553	CACCATAAATACCCTCAAACTGTTAGAG	28
R_sarS_638	TCATCTTCAGTTGAGCGTTCTTTT	24
P_sarS_596^B	AAAAGCAAGGCTATCTAA	18	FAM
*lytM*				SA0265
F_lytM_32	CGATGGGCTTCGCTACATTT	20
R_lytM_105	ATGTGCTTGTTGGGTGTTTGTC	22
P_lytM_56^A	TGGCGCATCAAGCAGATGCAGC	22	FAM
*sodA*				SA1382
F_sodA_168	TGTTGCTAATTTAGACAGTGTACCAGCTA	29
R_sodA_275	TCCCAGAATAATGAATGGTTTAAATG	26
P_sodA_198^A	CATCCAAACTGCTGTACGTAATAATGGCGG	30	FAM
*sirA*				SA0111
F_sirA_151	GGGAAACCAAAGCGTGTTGT	20
R_sirA_246	TGTCCATGATTCTACAGCACCTACA	25
P_sirA_198^A	ATATCAAGGTGCCACTGACGTCGCTGT	27	FAM

^A Taqman probes with a 3'-TAMRA quencher (Eurogentec, Seraing, Belgium).
^B Minor groove binder probe with non-fluorescent quencher bound to the 3'end (Applied Biosystems). All experiments used concentrations of 200 nM and 100 nM for primers and probes, respectively. Primers and probe for hla quantification have been published previously [87,88].

ISSN: 1471-2164