Comparison of miRNA expression between adult and aged lung tissue. A highly sensitive RT-PCR panel was used to analyse the expression profile of 256 miRNAs in adult (6 months) and aged (18 months) female BALB/c lung tissue. No significant changes in miRNA expression were observed in aged lung tissue (n = 5 for each age group). Data is represented as the mean ΔCT +/- S.E.M. (CT value of gene – CT value of 18 s). Higher expression is equivalent to a smaller ΔCT value. Statistical significance was measured using Student's t-test (p = 0.05) and data is representative of 5 animals.