Figure 6
Effect of exogenous H 2 O 2 on the expression of the putative ROS-mediated regulatory module that includes ROS-bZIP1 and representative genes that contain as1/ocs-like elements in their promoters ( OsMyb4 , high mobility group (HMG) protein, ferritin, and ATP sulfurylase). Rice seedlings (CT6748-8-CA-17) were subjected to a continuous 2 hours treatment with 1 mM H2O2. Transcript levels of H2O2-treated and non-treated (control) seedlings were monitored at specific time intervals during the 2 hours continuous H2O2 treatment and another 10 hours after the removal of the exogenous H2O2. Known H2O2-responsive gene that contains as1/ocs element-like motifs in its promoter (catalase) and a non-H2O2-responsive gene that do not contain as1/ocs-like motifs in its promoter (germin) were used as positive and negative controls, respectively. Transcript levels at each time point were normalized against the expression of constitutively expressed actin gene. Relative expression of control (0 hour; not shown in the graph) was set to zero by subtracting from the relative expression values of each treatment time points. Relative expression values are averages of independent replicates (n = 3; ± SE).