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Table 2 Chemostat cultivations of P. pastoris X33 producing 2F5 Fab utilizing the GAP promoter at two different temperatures.

From: Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions

 

YDM [g/L]

product [mg/L]

qP [mg g-1 h-1]

Chemostat 1

   

25°C

25.1

5.3

0.0079

20°C

23.2

7.4

0.0122

Chemostat 2

   

20°C

27.0

7.0

0.0091

25°C

24.8

5.2

0.0074

mean ratio 20°C/25°C

1.0

1.4

1.4

  1. Chemostat 1 was cultivated at 25°C first and temperature was lowered to 20°C after 5 volume changes, chemostat 2 was performed vice versa. Steady state conditions were assumed after 5 volume changes. Biomass (YDM), 2F5 Fab concentration (product) and specific production rate qP are given for both cultivations, additionally to the mean ratio of 20°C to 25°C for each of these parameters.
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BMC Genomics

ISSN: 1471-2164

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