The volcano plot for the female (A) and male (B) datasets. Volcano plot identifying genes which are significantly different between mature and immature female (A) and male (B) S. mansoni adult worms. The plot displays print-tip normalized fluorescence intensity ratios for all replicates on a two-axis system. The x-axis corresponds to the log2 of the fold change between mature and immature worms and the y-axis corresponds to the Log Odds (or B value) which is the odds (or probability) that a certain gene is differentially expressed. A Log Odds value of 0 (horizontal line in each graph) corresponds to a 50-50 chance that the gene is differentially expressed. The higher the Log Odds for each gene, the higher the probability that the gene is differentially expressed and not a false positive. The pink areas show genes that were considered as differentially expressed, i.e. it showed a fold difference greater than or equal to 2 (log2 fold change ≥ 1 or ≤ -1) and logOdds ≥ 4.6 (99% of chance of being statistically significant). The green area shows genes that have logOdds within the acceptable range but which have fold differences smaller than 2.