Figure 3

The dystrotelins and DAHs. Alignment of full-length sequences of dystrotelins ("Dyt") from a range of vertebrate species. Those from H. sapiens, M. musculus and D. rerio have been confirmed by RT-PCR and sequence analysis; the remainder are predicted from genomic sequence alone. Also included for context are the N-terminal sections of C. intestinalis dystrotelin-like protein, two insect DAH sequences, human dystrophin (Dp71 isoform; "Dys") and human α-dystrobrevin ("αDyb"; much of the C-terminal regions of these proteins are not alignable with dystrotelins and were omitted). Vertical lines indicate exon boundaries (the invertebrate genes are intronless). Lower case sequences in the primates indicate known (human) or expected (chimp, rhesus monkey) exclusion of the exons from the mRNA. Colour-coded boxes indicate known structural motifs – EF hands [48], ZZ domain [82] and coiled-coil regions predicted by COILS. Note three nonsense mutations (asterisks) in chimp sequence. Accession numbers: H. sapiens dystrotelin, [GenBank:DQ516347]; M. musculus dystrotelin, [GenBank:DQ443727]; D. rerio dystrotelin, [GenBank:DQ443726]; C. intestinalis dystrotelin, [GenBank:BR000175]; D. melanogaster DAH, [GenBank:AAC24159]; H. sapiens Dp71, [GenBank:M92650], H. sapiens α-dystrobrevin, [GenBank:NM_001390].