Figure 3

RT-PCR analysis of ACE3 mRNA expression in various murine tissues. Total RNA (1 μg) from the indicated tissues was subjected to one step RT-PCR as described in Materials and Methods followed by electrophoresis on a 1% agarose gel. (A) mouse ACE3; (B) actin. Lanes are as follows: 1, positive control (whole mouse RNA with primers for actin); 2, negative control (no RNA); 3, brain; 4, embryo; 5, heart; 6, kidney; 7, liver; 8, lung; 9, ovary; 10, spleen; 11, testis; 12, thymus.