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Table 1 Sequences of adaptors and the adaptor primer.

From: A novel technique for measuring variations in DNA copy-number: competitive genomic polymerase chain reaction

Name

Oligomer

Sequences

PT adaptors

  

PT1

  
 

bPT1L

5'-biotin-GTACATATTGTCGTTAGAACGCG TCAATCCATACTTGCA-3'

 

PT1S

5'-AGTATGGATTGACGCGTTCTAACGACAATATGTAC-3'

PT2

  
 

bPT2L

5'-biotin-GTACATATTGTCGTTAGAACGCG TACTCAATCCATACTTGCA-3'

 

PT2S

5'-AGTATGGATTGAGTACGCGTTCTAACGACAATATGTAC-3'

PT3

  
 

bPT3L

5'-biotin-GTACATATTGTCGTTAGAACGCG CTATACTCAATCCATACTTGCA-3'

 

PT3S

5'-AGTATGGATTGAGTATAGCGCGTTCTAACGACAATATGTAC-3'

Adaptor primer

  

C1S-FAM

 

5'-6FAM -GTACATATTGTCGTTAGAACGC-3'

  1. Each adaptor consists of a pair of oligomers designated S and L. The three adaptors shared a common sequence (bold) and had a cohesive end complementary to the PstI restriction site (underlined). Biotin was added to the 5' ends of the longer oligomers for the subsequent purification step, and 6FAM was used to label the C1S adaptor primer, which was complementary to the shared sequence of the three adaptors.
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BMC Genomics

ISSN: 1471-2164

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