Figure 3

Lineage specific expansion of the Daphnia pulex ferritin genes. Neighbor-Joining (NJ) tree inferred from the deduced amino acid sequences of the Daphnia ferritin genes, including loci from Drosophila melanogaster plus other representative insect and crustacean amino acid sequences obtained from the NCBI and FlyBase protein sequence repositories. Ferritin 1 group contains insect and Daphnia Fer1HCH gene(s). Ferritin 2 group only contains insect Fer2LCH loci and the Ferritin 3 group contains insect and crustacean Fer3HCH genes. The amino acid sequence alignment was obtained by using t-coffee [72] and is available by request. The NJ tree was constructed using MEGA3 [73] using the Poisson correction for calculating the distance matrix. The bootstrap support values are shown at the main branch nodes of the tree, which are derived from 1000 pseudo-replication of the data. D. pulex sequences denoted by * were obtained from an ongoing cDNA sequencing project by the Joint Genome Institute and the Daphnia Genomics Consortium (Colbourne et al. in prep) and are deposited in Genbank under accession numbers DQ983425-DQ983438. GenInfo (GI) accessions for all other sequences: 6946692; 61744051; 26006755; 46561742; 91081285; 87083910; 66504201; 1807496; 13195275; 55242312; 66524157; 91077442; 24651358; 95702694; 18031707; 62722854; 6409191; 91077446; 66524161; 7272336; 62722856.