Table 2 Representative Bvg- phase specific genes identified by microarray analysis.
Function | Gene | Product | Fold-Change ± SEM |
|---|---|---|---|
Cell Adhesin | BB1186 | putative hemolysin, homophilic cell adhesion | -2.83 ± 0.18 |
Cell Envelope | BB0141 wbmD | putative membrane protein | -5.19 ± 0.08 |
|  | BB2618 flgJ | peptidoglycan hydrolase | -28.12 ± 0.01 |
|  | BB2618 lpxB | lipid-A-disaccharide synthase; lipid A biosynthesis | -6.35 ± 0.02 |
|  | BB2879 | putative membrane protein | -6.46 ± 0.02 |
|  | BB4842 | putative outer membrane protein | -32.02 ± 0.01 |
Chemotaxis | BB2543 motA | chemotaxis protein MotA | -26.28 ± 0.02 |
|  | BB2544 motB | chemotaxis protein MotB | -17.90 ± 0.02 |
|  | BB2549 cheR | chemotaxis protein methyltransferase | -10.43 ± 0.03 |
|  | BB2552 cheZ | chemotaxis protein CheZ; catalytic activity | -38.44 ± 0.01 |
Motility | BB2553 flhB | flagellar biosynthetic protein FlhB | -44.48 ± 0.01 |
|  | BB2555 flhF | flagellar biosynthesis protein; RNA binding | -32.10 ± 0.00 |
|  | BB2559 flgB | flagellar basal-body rod protein FlgB; motor activity | -44.49 ± 0.01 |
|  | BB2560 flgC | flagellar basal-body rod protein FlgC; motor activity | -32.10 ± 0.00 |
|  | BB2585 fliI | flagellum-specific ATP synthase FliI; ATP binding | -30.75 ± 0.01 |
Electron Transport | BB1283 cyoA | ubiquinol oxidase polypeptide II; copper ion binding | -5.11 ± 0.08 |
|  | BB2800 napA | periplasmic nitrate reductase precursor; iron ion binding | -11.34 ± 0.01 |
|  | BB3325 | putative ferredoxin; electron transport activity | -32.43 ± 0.00 |
|  | BB3927 | putative cytochrome; electron transport activity | -5.58 ± 0.01 |
|  | BB4096 | putative oxidoreductase | -4.90 ± 0.02 |
|  | BB4497 cydB | cytochrome D ubiquinol oxidase subunit II | -6.04 ± 0.02 |
|  | BB4498 cydA | cytochrome D ubiquinol oxidase subunit I | -5.40 ± 0.07 |
|  | BB4945 ivd | isovaleryl-CoA dehydrogenase | -8.90 ± 0.01 |
Protein Folding and Ushering | BB0979 | putative universal stress protein | -24.76 ± 0.02 |
|  | BB2875 | universal stress family protein | -7.45 ± 0.01 |
|  | BB3257 fkpB | FkbP-type peptidyl-prolyl cis-trans isomerase | -4.15 ± 0.01 |
|  | BB4260 | putative universal stress protein | -9.29 ± 0.01 |
Global Regulatory Functions | BB0725 | putative transcriptional regulator | -7.82 ± 0.04 |
|  | BB1122 | two-component system sensor kinase; signal transducer | -9.65 ± 0.01 |
|  | BB1187 | putative LuxR-family transcriptional regulator | -11.60 ± 0.02 |
|  | BB2108 | probable two-component response regulator | -18.08 ± 0.01 |
|  | BB2323 | putative transcriptional regulator | -11.03 ± 0.03 |
|  | BB2540 fliA | RNA polymerase sigma factor; flagellar operon | -16.05 ± 0.01 |
|  | BB2542 flhC | flagellar transcriptional activator FlhC; DNA binding | -31.06 ± 0.01 |
|  | BB2550 cheB | protein-glutamate methylesterase;two-component regulator | -18.56 ± 0.02 |
|  | BB3115 | methyl-accepting chemotaxis signal transducer protein | -10.74 ± 0.02 |
|  | BB3866 | probable LysR-family transcriptional regulator | -7.15 ± 0.02 |
Metabolism | BB0868 mmsA | methylmalonate-semialdehyde dehydrogenase | -8.72 ± 0.02 |
|  | BB0978 ggt | gamma-glutamyltranspeptidase precursor | -35.18 ± 0.02 |
|  | BB1367 cysG | siroheme synthase, heme biosynthesis | -8.67 ± 0.01 |
|  | BB2085 hemC | porphobilinogen deaminase; synthase activity | -16.39 ± 0.01 |
|  | BB2147 hemN | coproporphyrinogen III oxidase | -5.84 ± 0.01 |
|  | BB4409 hemL | glutamate-1-semialdehyde 2,1-aminomutase | -13.10 ± 0.01 |
Transport | BB0976 | lactate permease family protein | -21.29 ± 0.01 |
|  | BB1174 | putative ABC transport proteins; ATP-binding component | -5.52 ± 0.03 |
|  | BB1188 | HlyD-family secretion protein; protein transporter activity | -6.27 ± 0.05 |
|  | BB1189 | probable ABC transporter | -7.56 ± 0.06 |
|  | BB1191 | putative outer membrane protein; transporter activity | -13.87 ± 0.02 |
|  | BB1294 bfrG | putative TonB-dependent receptor | -2.86 ± 0.03 |
|  | BB1942 bfeA | ferric enterobactin receptor | -2.80 ± 0.02 |
|  | BB2402 | putative sulfate transporter | -6.92 ± 0.02 |
|  | BB2433 | multidrug resistance protein | -4.14 ± 0.03 |
|  | BB2803 ccmA | putative heme export protein; heme transporter activity | -12.62 ± 0.01 |
|  | BB2804 ccmB | heme exporter protein B | -9.57 ± 0.01 |
|  | BB2805 ccmC | putative heme export protein | -13.01 ± 0.01 |
|  | BB4273 atoE | putative short-chain fatty acids transporter | -7.49 ± 0.01 |
|  | BB4495 | probable ATP-binding component; ABC transporter | -33.13 ± 0.01 |