The phenotype of An. gambiae in response to coinjection of ONNV-eGFP and dsHSC70B. (A) Characterization of viral transcripts of ONNV-eGFP after coinjection with dsβ-gal into adult female An. gambiae (4arr strain). Ctr, 3 and 6 represent transcript profile of the recombinant ONNV with enhanced green fluorescent protein (ONNV-eGFP) before, at 3 and 6 days p.i., respectively. Primers for RT-PCR were designed from the ONNV structural E1 gene (E1) and non-structural protein 1 gene (nsP1) (Table 1). The PCR products of the E1 and nsP1 primer pairs show the expected sizes, 431 and 440 base pairs, respectively (Table 1), indicating the correct expression of ONNV-eGFP in An. gambiae. (B) The strong expression of GFP in head, thorax, and abdomen of An. gambiae at 6 days after coinjected with dsHSC70B and ONNV-eGFP. (C) The relatively weak expression of GFP in the thorax of An. gambiae at 6 days after coinjected with dsβ-gal and ONNV-eGFP. The arrows indicate the tissues of An. gambiae with GFP expression.