Figure 2

Evolutionary conservation of the DNA-binding regions. The alignment of fungal and selected metazoan CSL protein sequences (see Table 1 and Additional file 3 for details) shows high degree of conservation in regions responsible for DNA binding. Absolutely conserved residues are inverse-printed, positions with high residue similarity are boxed. Domain boundaries are indicated by color: green for RHR-N, red for BTD and blue for the βC4 linker connecting all three CSL domains. Red and cyan triangles below the alignment denote residues required for sequence specific and backbone DNA binding, respectively. The position numbering and secondary structures indicated above the sequences correspond to C. elegans LAG-1 [17]. The picture shows only a selected region of the whole alignment and, in order to save space, some parts of the long inserts are not shown (indicated by '//'). The picture was created using ESPript [50].