Figure 4
From: Use of tiling array data and RNA secondary structure predictions to identify noncoding RNA genes
Experimental verification of CRUFTS showing high RNAz SVM probability. A) Histogram showing enrichment of known ncRNAs in the top 300 CRUFTS sorted on RNAz SVM probability B) Northern blots with specific LNA modified DNA probes for three high covariance CRUFTS. The U68 snoRNA is the positive control. C) Alignment and conserved secondary structure of the CRUFTS C3462. The location of the probe used for detection is indicated. The positions in the alignments and the secondary structure are color-coded according to the conservation of the basepair interaction following the RNAz conventions [9]. Green indicates that 3 different types of pairs (e.g. G-C in human, G-U in dog and A-U in zebrafish) support the interaction. Yellow color coding indicates that the base pair is supported by 2 types of pairs and red that only a single pair-type supports the interaction. The intensity of color coding is faded fates with the number of sequences in conflict with the predicted interaction.