Figure 8

Pterin utilization by an E. coli folE deletant harboring Xylella fastidiosa PD0677 or Leishmania major PTR1. Deletant cells transformed with pBluescript (pBS) alone, or pBS harboring Xylella fastidiosa Temecula1 PD0677 or Leishmania major PTR1, were streaked on LB medium containing IPTG and appropriate antibiotics, without or with 300 μM thymidine (dT) or 19 μM 6-hydroxymethylpterin (HMPt). For PD0677, neopterin, monapterin, and pterin-6-aldehyde were also tested and found not to support growth (not shown). 1, pBS::PD0677; 2, pBS; 3, pBS::PTR1.