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Table 4 Genes that have a high confidence in congruence between protein and transcript profile during growth

From: New pleiotropic effects of eliminating a rare tRNA from Streptomyces coelicolor, revealed by combined proteomic and transcriptomic analysis of liquid cultures

Gene/spot

Confidence ((1-p) × 100)

Spearman rank correlation

Annotated gene function

Positive correlation in set A

SCO0379:123

100

1

catalase KatA

SCO1651

100

1

conserved hypothetical protein

SCO1776

100

1

putative CTP synthetase PyrG

SCO2554

100

1

DnaJ

SCO7510

100

1

peptidyl-prolyl cis-trans isomerase CypH

Positive correlation in set B

SCO1212

100

1

putative ligase

SCO2115

100

1

putative 2-dehydro-3-deoxyphosphoheptonate aldolase AroH

SCO2539

100

1

Era-like GTP-binding protein

SCO3801

100

1

putative aminopeptidase

SCO3907

100

1

single strand DNA-binding protein ssb

SCO4809

100

1

succinyl CoA synthetase alpha chain SucD

SCO4813

97.7

0.87

phosphoribosylglycinamide formyltransferase PurN

SCO4824

98.4

0.89

bifunctional protein (methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase FolD

SCO4921

100

1

putative acyl-CoA carboxylase complex A subunit AccA2

SCO5745

100

1

conserved hypothetical protein

Positive correlation in set C

SCO0379:123

97

0.85

catalase KatA

SCO1244

98

0.88

Biotin synthase BioB

SCO1489

98.4

0.89

DNA-binding protein BldD

SCO1523

98.4

0.92

conserved hypothetical protein

SCO1651

98.4

0.89

conserved hypothetical protein

SCO1776

99.8

0.96

putative CTP synthetase PyrG

SCO2949

98

0.88

UDP-N-acetylglucosamine transferase MurA

SCO3122

98.4

0.9

putative nucleotidyltransferase

SCO4550

98.4

0.9

conserved hypothetical protein

SCO4813

97.7

0.87

phosphoribosylglycinamide formyltransferase PurN

SCO4824

98.4

0.89

bifunctional protein (methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase FolD

SCO5113

98.4

0.9

BldKB, putative ABC transport system lipoprotein,

Negative correlation (all in set C)

SCO1947:683

98.4

-0.86

glyceraldehyde-3-phosphate dehydrogenase Gap1

SCO2198:235

97.7

-0.87

glutamine synthetase I GlnA

SCO2198:746

98.8

-0.92

glutamine synthetase I GlnA

SCO2634

97

-0.85

conserved hypothetical protein

SCO2736:472

95.1

-0.83

Citrate synthase CitA

SCO5466

97.8

-0.87

putative hydrolase

  1. Congruence across time was calculated by Spearman Rank Correlation given different data sets where set A represents 5 time points in M600, B represents 5 time points in ΔbldA and C represents 10 time points spanning both M600 and ΔbldA (the 5 time points are 1, 2, 4, 6 and 8 in Fig. 1).