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Archived Comments for: WASP: a Web-based Allele-Specific PCR assay designing tool for detecting SNPs and mutations

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  1. query regarding this software

    Mamoonah Chaudhry, university of the punjab

    18 October 2011

    dear author,
    one of my friend referred this article to me as i was aiming to do allele specific PCR. i found this software very useful.As i am trying to design primers using this software, maximum Tm difference is quite confusing, as far as my knowledge is concerned, it should be not more than 5 degree c. whenever i try to change it in software, it designs no primers. please give me your email address for direct contact or please help me in this regard.


    Competing interests

    None declared