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Table 5 PRI-lock assay validation. Analysis of target DNAs mixed in different ratios.

From: Quantitative multiplex detection of plant pathogens using a novel ligation probe-based system coupled with universal, high-throughput real-time PCR on OpenArrays™

Template (Log10 target
(copy number/μl ligation reaction))
Ratio Observed CT values (SD) in PRI-lock specific PCR Observed pathogen template (SD)
(Log10 target (copy number/μl ligation reaction))
P. sojae E. carotovora carotovora   Phytophthora spp. E. carotovora carotovora Phytophthora spp. E. carotovora carotovora
8 5 1000: 1 9.4 (0.30) 19.3 (0.23) 7.9 (0.08) 4.9 (0.07)
8 4 10000: 1 9.3 (0.06) 22.7 (0.34) 8.0 (0.02) 3.9 (0.10)
5 8 1: 1000 20.6 (0.20) 8.8 (0.14) 4.8 (0.06) 7.9 (0.04)
4 8 1: 10000 24.2 (0.80) 8.9 (0.07) 3.8 (0.22) 7.8 (0.02)
  1. Target numbers were calculated using the CT values and the corresponding calibration curves. Data represent 2 ligation replicates (n = 2), each with 4 qPCR replicates (n = 4).