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Table 6 PRI-lock assay validation. Analysis of genomic DNA targets mixed in different ratios.

From: Quantitative multiplex detection of plant pathogens using a novel ligation probe-based system coupled with universal, high-throughput real-time PCR on OpenArrays™

Genomic DNA template (pg/μl ligation mixture) Log10 target (copy number/μl ligation mixture) (SD)
F. oxysporum P. fragariae M. roridum R. solani AG 4-1 R. solani AG 4-2 E. c. c. F. oxysporum P. fragariae M. roridum R. solani AG 4-1 R. solani AG 4-2 E. c. c. Gamma Proteo bacterial spp.
100 100 100 -- -- 10 6.3 (0.10) 6.8 (0.08) 6.8 (0.07) -- -- 3.5 (0.09) 4.6 (0.10)
1 0.1 100 -- -- -- 4.2 (0.12) 3.6 (0.27) 6.7 (0.05) -- -- -- --
100 0.01 0.01 -- -- 100 6.1 (0.14) nd nd -- -- 4.4 (0.08) 5.5 (0.13)
10 100 1 -- -- 1 5.1 (0.08) 6.8 (0.09) 4.4 (0.11) -- -- 2.8 (0.17) 3.5 (0.11)
10 0.01 1 -- -- 10 5.2 (0.17) nd 4.5 (0.04) -- -- 3.5 (0.20) 4.5 (0.16)
10 0.001 1 -- -- 0.01 5.1 (0.09) nd 4.4 (0.20) -- -- nd nd
10 1 0.1 -- -- 100 5.1 (0.15) 4.6 (0.14) 3.3 (0.04) -- -- 4.4 (0.07) 5.4 (0.05)
10 10 10 10 10 10 5.0 (0.07) 5.6 (0.05) 5.4 (0.06) 4.3 (0.08) 5.0 (0.20) 3.2 (0.23) 4.3 (0.08)
-- -- -- 100 1 -- -- -- -- 5.7 (0.06) 4.0 (0.23) -- --
0.1 -- -- 1 -- 10 3.8 (0.20) -- -- 3.3 (0.16) -- 3.5 (0.10) 4.6 (0.10)
  1. Target numbers were calculated using the CT values and the corresponding calibration curves. Data highlighted in bold font, indicate data outside the linear range. Data represent 2 ligation replicates (n = 2), each with 4 qPCR replicates (n = 4). Nd: not detectable. E.c.c.:Erwinia carotovora carotovora