Table 6 PRI-lock assay validation. Analysis of genomic DNA targets mixed in different ratios.
Genomic DNA template (pg/μl ligation mixture) | Log10 target (copy number/μl ligation mixture) (SD) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
F. oxysporum | P. fragariae | M. roridum | R. solani AG 4-1 | R. solani AG 4-2 | E. c. c. | F. oxysporum | P. fragariae | M. roridum | R. solani AG 4-1 | R. solani AG 4-2 | E. c. c. | Gamma Proteo bacterial spp. |
100 | 100 | 100 | -- | -- | 10 | 6.3 (0.10) | 6.8 (0.08) | 6.8 (0.07) | -- | -- | 3.5 (0.09) | 4.6 (0.10) |
1 | 0.1 | 100 | -- | -- | -- | 4.2 (0.12) | 3.6 (0.27) | 6.7 (0.05) | -- | -- | -- | -- |
100 | 0.01 | 0.01 | -- | -- | 100 | 6.1 (0.14) | nd | nd | -- | -- | 4.4 (0.08) | 5.5 (0.13) |
10 | 100 | 1 | -- | -- | 1 | 5.1 (0.08) | 6.8 (0.09) | 4.4 (0.11) | -- | -- | 2.8 (0.17) | 3.5 (0.11) |
10 | 0.01 | 1 | -- | -- | 10 | 5.2 (0.17) | nd | 4.5 (0.04) | -- | -- | 3.5 (0.20) | 4.5 (0.16) |
10 | 0.001 | 1 | -- | -- | 0.01 | 5.1 (0.09) | nd | 4.4 (0.20) | -- | -- | nd | nd |
10 | 1 | 0.1 | -- | -- | 100 | 5.1 (0.15) | 4.6 (0.14) | 3.3 (0.04) | -- | -- | 4.4 (0.07) | 5.4 (0.05) |
10 | 10 | 10 | 10 | 10 | 10 | 5.0 (0.07) | 5.6 (0.05) | 5.4 (0.06) | 4.3 (0.08) | 5.0 (0.20) | 3.2 (0.23) | 4.3 (0.08) |
-- | -- | -- | 100 | 1 | -- | -- | -- | -- | 5.7 (0.06) | 4.0 (0.23) | -- | -- |
0.1 | -- | -- | 1 | -- | 10 | 3.8 (0.20) | -- | -- | 3.3 (0.16) | -- | 3.5 (0.10) | 4.6 (0.10) |