Figure 3

Principal component analysis of 12 individual hybridizations. Each hybridization result is illustrated as a colored circle in a 3D graph. The similar the color, the similar the sample. The 2^nd, 3^rd and 4^th components are demonstrated on the x-, y- and z-axis respectively with negative and positive values. The 2^nd component explains differences in expression of tumor and bronchus (negative values (red-orange) are bronchus, positive values (blue-green) are tumor samples). The 3^rd component explains the differences in number of amplification rounds (negative values (orange and green) are one round amplified, positive values (red and blue) are two rounds amplified) and the 4^th component explains the labeling type of Cy-dye (negative values (light colored) are Cy3 labeled, positive values (dark colored) are Cy5 labeled samples).